期刊
ADVANCED HEALTHCARE MATERIALS
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202303672
关键词
cell morphology/shape; collagen alignment; microfluidics; phenotype maintenance; tenocytes
Tendons are highly organized tissues that require aligned collagen fibers for proper function. This study developed a microfluidic platform that allows controlled formation and alignment of collagen fibers, leading to changes in cell morphology and marker expression in tenocytes. This research has implications for tendon regeneration and the development of physiologically relevant in vitro models.
Tendon is a highly organized tissue that transmits forces between muscle and bone. The architecture of the extracellular matrix of tendon, predominantly from collagen type I, is important for maintaining tenocyte phenotype and function. Therefore, in repair and regeneration of damaged and diseased tendon tissue, it is crucial to restore the aligned arrangement of the collagen type I fibers of the original matrix. To this end, a novel, user-friendly microfluidic piggyback platform is developed allowing the controlled patterned formation and alignment of collagen fibers simply on the bottom of culture dishes. Rat tenocytes cultured on the micropatterns of aligned fibrous collagen exhibit a more elongated morphology. The cells also show an increased expression of tenogenic markers at the gene and protein level compared to tenocytes cultured on tissue culture plastic or non-fibrillar collagen coatings. Moreover, using imprinted polystyrene replicas of aligned collagen fibers, this work shows that the fibrillar structure of collagen per se affects the tenocyte morphology, whereas the biochemical nature of collagen plays a prominent role in the expression of tenogenic markers. Beyond the controlled provision of aligned collagen, the microfluidic platform can aid in developing more physiologically relevant in vitro models of tendon and its regeneration. A user-friendly microfluidic piggyback platform is developed allowing the controlled patterned formation and alignment of collagen fibers simply on the bottom of culture dishes. Rat tenocytes cultured on the micropatterns of aligned fibrous collagen exhibit a more elongated morphology and show an increased expression of tenogenic markers compared to tenocytes on tissue culture plastic or non-fibrillar collagen coatings.image
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