期刊
ADVANCED HEALTHCARE MATERIALS
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202301815
关键词
3-bromopyruvate; cancer microenvironment; glycolysis; lipid metabolism; pancreatic cancer; Raman spectroscopy; Warburg effect
This study demonstrates the simultaneous profiling of three protein biomarkers using SERS nanotags and antibody-functionalized nanoparticles in a syngeneic mouse model of pancreatic cancer. The multimodal imaging techniques used provide comprehensive information about biomarkers and TAM alterations before and after treatment. The study reveals the efficacy of 3-BP in treating pancreatic cancer and identifies drug treatment-induced lipid species remodeling and associated pathways through bioinformatics analysis.
Lipid metabolism and glycolysis play crucial roles in the progression and metastasis of cancer, and the use of 3-bromopyruvate (3-BP) as an antiglycolytic agent has shown promise in killing pancreatic cancer cells. However, developing an effective strategy to avoid chemoresistance requires the ability to probe the interaction of cancer drugs with complex tumor-associated microenvironments (TAMs). Unfortunately, no robust and multiplexed molecular imaging technology is currently available to analyze TAMs. In this study, the simultaneous profiling of three protein biomarkers using SERS nanotags and antibody-functionalized nanoparticles in a syngeneic mouse model of pancreatic cancer (PC) is demonstrated. This allows for comprehensive information about biomarkers and TAM alterations before and after treatment. These multimodal imaging techniques include surface-enhanced Raman spectroscopy (SERS), immunohistochemistry (IHC), polarized light microscopy, second harmonic generation (SHG) microscopy, fluorescence lifetime imaging microscopy (FLIM), and untargeted liquid chromatography and mass spectrometry (LC-MS) analysis. The study reveals the efficacy of 3-BP in treating pancreatic cancer and identifies drug treatment-induced lipid species remodeling and associated pathways through bioinformatics analysis. Simultaneous profiling of lipids, collagen, and proteins is challenging using a single immunohistochemistry marker. The rational design of surface-enhanced Raman spectroscopy (SERS) nanotags in the Raman-silent region helps us to perform spatial mapping of multiple proteins and lipids in the pancreatic tumor model after treatment.image
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据