期刊
VIRULENCE
卷 14, 期 1, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21505594.2023.2232707
关键词
African swine fever virus; p17; mitophagy; TOMM70; SQSTM1
African swine fever virus (ASFV) uses its protein p17 to induce mitophagy, leading to the degradation of mitochondria and inhibition of host innate immunity.
Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-& alpha;, IL-6 and TNF & alpha;. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70.
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