期刊
NANO LETTERS
卷 16, 期 4, 页码 2686-2691出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.6b00331
关键词
Caspase 3/7; aggregation; ultrasmall superparamagnetic iron oxide; transverse relaxation; magnetic resonance imaging
类别
资金
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- Major Program of Development Foundation of Hefei Center for Physical Science and Technology
- Hefei Science Center CAS [2015HSC-UP012]
- Fundamental Research Funds for the Central Universities [WK2060190054]
- National Natural Science Foundation of China [U1532144, 21375121, U1232212, U1332142]
Large magnetic nanoparticles or aggregates are advantageous in their magnetic resonance properties over ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles (NPs), but the former are cleared faster from the blood pool. Therefore, the smart strategy of intracellular aggregation of USPIO NPs is required for enhanced T-2-weighted MR imaging. Herein, employing an enzyme-instructed condensation reaction, we rationally designed a small molecule Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys-CBT (1) to covalently modify USPIO NPs to prepare monodispersive Fe3O4@1 NPs. In vitro results showed that Fe3O4@1 NPs could be subjected to caspase 3 (Casp3)-instructed aggregation. T-2 phantom MR imaging showed that the transverse molar relaxivity (r(2)) of Fe3O4@1 NPs with Casp3 or apoptotic HepG2 cells was significantly larger than those of control groups. In vivo tumor MR imaging results indicated that Fe3O4@1 NPs could be specifically applied for enhanced T-2 MR imaging of tumor apoptosis. We propose that the enzyme-instructed intracellular aggregation of Fe3O4 NPs could be a novel strategy for the design of smart probes for efficient T-2 MR imaging of in vivo biomarkers.
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