Recruitment and migration patterns reveal a key role for seed banks in the meta-population dynamics of an aquatic plant

Progressive habitat fragmentation threatens plant species with narrow habitat requirements. While local environmental conditions define population growth rates and recruitment success at the patch level, dispersal is critical for population viability at the landscape scale. Identifying the dynamics of plant meta-populations is often confounded by the uncertainty about soil-stored population compartments. We combined a landscape-scale assessment of an amphibious plant's population structure with measurements of dispersal complexity in time to track dispersal and putative shifts in functional connectivity. Using 13 microsatellite markers, we analyzed the genetic structure of extant Oenanthe aquatica populations and their soil seed banks in a kettle hole system to uncover hidden connectivity among populations in time and space. Considerable spatial genetic structure and isolation-by-distance suggest limited gene flow between sites. Spatial isolation and patch size showed minor effects on genetic diversity. Genetic similarity found among extant populations and their seed banks suggests increased local recruitment, despite some evidence of migration and recent colonization. Results indicate stepping-stone dispersal across adjacent populations. Among permanent and ephemeral demes the resulting meta-population demography could be determined by source-sink dynamics. Overall, these spatiotemporal connectivity patterns support mainland-island dynamics in our system, highlighting the importance of persistent seed banks as enduring sources of genetic diversity.

Automated summary

Progressive habitat fragmentation threatens plant species with narrow habitat requirements. Dispersal is critical for population viability at the landscape scale, but is often confounded by the uncertainty about soil-stored population compartments. The study analyzed the genetic structure of Oenanthe aquatica populations and their soil seed banks to uncover hidden connectivity among populations in time and space. Results indicate stepping-stone dispersal across adjacent populations and suggest the importance of persistent seed banks as enduring sources of genetic diversity.