Vitamin A Promotes the Repair of Mice Skeletal Muscle Injury through RARa

Vitamin A (VitA) is an important fat-soluble vitamin which plays an important role in cell growth and individual development. However, the effect of VitA on the repair process of muscle injury and its molecular mechanism are still unclear. In this study, VitA and RA were first added to the culture medium of differentiated cells. We then detected cell differentiation marker proteins and myotube fusion. Moreover, the effects of VitA on RARa expression and nuclear translocation were further examined. The results showed that VitA significantly promoted the differentiation of C2C12, and the expression of RARa was significantly increased. Furthermore, VitA was injected into skeletal muscle injury in mice. HE staining and Western Blot results showed that VitA could significantly accelerate the repair of skeletal muscle injury and VitA increase the expression of RARa in mice. This study provides a theoretical basis for elucidating the regulation mechanism of VitA-mediated muscle development and the development of therapeutic drugs for muscle diseases in animals.

Automated summary

Vitamin A is important for cell growth and development, but its role in muscle injury repair and molecular mechanism are unclear. This study found that Vitamin A promotes differentiation of C2C12 cells and increases RARa expression. In a mouse model of skeletal muscle injury, Vitamin A significantly accelerated repair and increased RARa expression. The study provides a theoretical basis for understanding Vitamin A-mediated muscle development and developing therapeutic drugs for muscle diseases in animals.