DNA barcoding is an effective tool for differentiating Pisolithus species from Macedonia

With the aim of clarifying the number of Pisolithus species in Macedonia, molecular analysis was carried out on seven specimens collected in oak forests in Macedonia during 1987 and 2005. New sequences were compared with previously published sequences included in public databases. Three Pisolithus species are identified in Macedonia: P. arhizus, P. capsulifer, and P. tinctorius.