期刊
MUSCLE & NERVE
卷 55, 期 3, 页码 373-383出版社
WILEY
DOI: 10.1002/mus.25225
关键词
mRNA; microarray; peripheral nerve injury; regeneration; Schwann cell; Wallerian degeneration
资金
- State Key Program of the National Natural Science Foundation of China [81330042]
- Special Program for Sino-Russian Joint Research of the Ministry of Science and Technology, China [2014DFR31210]
- Tianjin Science and Technology Committee, China [13RCGFSY19000, 14ZCZDSY00044]
Introduction: The purpose of this study was to provide a comprehensive understanding of gene expression during Wallerian degeneration and axon regeneration after peripheral nerve injury. Methods: A microarray was used to detect gene expression in the distal nerve 0, 3, 7, and 14 days after sciatic nerve crush. Bioinformatic analysis was used to predict function of the differentially expressed mRNAs. Microarray results and the key pathways were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: Differentially expressed mRNAs at different time-points (3, 7, and 14 days) after injury were identified and compared with a control group (0 day). Nine general trends of changes in gene expression were identified. Key signal pathways and 9 biological processes closely associated with nerve regeneration were identified and verified. Conclusions: Differentially expressed genes and biological processes and pathways associated with axonal regeneration may elucidate the molecular-biological mechanisms underlying peripheral nerve regeneration. Muscle Nerve55: 373-383, 2017
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