期刊
MBIO
卷 -, 期 -, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/mbio.01506-23
关键词
colistin; Pseudomonas aeruginosa; microfluidics; experimental evolution; cystic fibrosis
类别
Antibiotic resistance is a global health crisis, and understanding the evolutionary trajectories leading to increased resistance is crucial for developing clinical diagnostics and discovering new drug targets. Researchers found that adaptive mutations to phoQ were more successful than pmrB in increasing resistance to colistin, a commonly used antibiotic. The study also revealed that the oxygen concentration gradients within the microdroplet emulsions favored changes to the PhoP/PhoQ pathway, which is consistent with the microaerobic conditions found in the lungs of cystic fibrosis patients.
Antibiotic resistance is a continuing global health crisis. Identifying the evolutionary trajectories leading to increased antimicrobial resistance can be critical to the discovery of biomarkers for clinical diagnostics and new targets for drug discovery. While the combination of patient data and in vitro experimental evolution has been remarkably successful in extending our understanding of antimicrobial resistance, it can be difficult for in vitro methods to recapitulate the spatial structure and consequent microenvironments that characterize in vivo infection. Notably, in cystic fibrosis (CF) patients, changes to either the PmrA/PmrB or PhoP/PhoQ two-component systems have been identified as critical drivers for high levels of colistin and polymyxin resistance. When using microfluidic emulsions to provide spatially structured, low-competition environments, we found that adaptive mutations to phoQ were more successful than pmrB in increasing colistin resistance. Conversely, mutations to pmrB were readily identified using well-mixed unstructured cultures. We found that oxygen concentration gradients within the microdroplet emulsions favored adaptive changes to the PhoP/PhoQ pathway consistent with microaerobic conditions that can be found in the lungs of CF patients. We also observed mutations linked to hallmark adaptations to the CF lung environment, such as loss of motility and loss of O antigen biosynthesis (wbpL). Mutation to wbpL, in addition to causing loss of O antigen, was additionally shown to confer moderately increased colistin resistance. Taken together, our data suggest that distinct evolutionary trajectories to colistin resistance may be shaped by the microaerobic partitioning and spatial separation imposed within the CF lung.
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