Re-evaluation of the systematic position of the order Prostomatida (Protista: Ciliophora), with the establishment of two new genera and two new species

Prostomatean ciliates inhabit a wide range of aquatic environments and serve as a trophic link in food webs. However, the biodiversity and molecular phylogeny of the Prostomatea, especially the order Prostomatida, is poorly known owing to undersampling and the paucity of molecular data. Here, three prostomatid ciliates, Platina marina gen. nov., sp. nov., Parametacystis pulchra gen. nov., sp. nov. and Apsiktrata gracilis, were studied based on morphological data and small subunit ribosomal ribonucleic acid (SSU rRNA) gene sequences. The two new genera show a close relationship with the class Plagiopylea but a distant relationship with Metacystis, which bears a strong morphological similarity to the two new genera. The simplified oral ciliature can be regarded as a synapomorphy of the two new genera. The SSU rRNA gene of a representative of the family Apsiktratidae was sequenced for the first time. Morphological and phylogenetic analyses suggest that Apsiktratidae might be more closely related to Prorodontida than to Prostomatida, and the oral basket might be more phylogenetically informative than the brosse in distinguishing Prorodontida and Prostomatida. Supplementation with rare taxa reveals a close association between the Prostomatida and Trimyemidae. The putative secondary structure of the SSU rRNA V9 region suggests a sister relationship between the order Prostomatida and the class Plagiopylea.

Automated summary

This study investigates the biodiversity and molecular phylogeny of prostomatean ciliates, particularly the order Prostomatida. Based on morphological data and SSU rRNA gene sequences, three new prostomatid ciliates were discovered, along with their relationship to other taxa. The study also suggests that the simplified oral ciliature is a characteristic feature of the new genera. Furthermore, a close association between Prostomatida and Trimyemidae was revealed.