Lysis and Concentration Technique improves the parasitological diagnosis of Trypanosoma vivax

The Lysis and Concentration Technique (LCTe) involves lysing red blood cells and concentrating parasites to increase the chances of visualization in low parasitemia and in scenarios of evaluators with less knowledge. The lysis of red blood cells reduced the time of diagnosis by 21 s, showing that the effect produced by the treatment is comparable to the effect of the experience in parasitological examination. In addition, the concentration of parasites was 39.18% higher for slides with high parasitemia and 131.03% for slides with low parasitemia in relation to the standard slide. LCTe proved to be inexpensive, with a total cost of approximately US$0.07 per slide made, which allows it to be easily implemented in most laboratories.

Automated summary

The Lysis and Concentration Technique (LCTe) involves lysing red blood cells and concentrating parasites to increase visualization in cases of low parasitemia and limited knowledge. Lysis of red blood cells reduced diagnosis time and the concentration of parasites increased significantly compared to the standard slide. LCTe is a cost-effective method that can be easily implemented in most laboratories.