期刊
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
卷 261, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.vetimm.2023.110611
关键词
Dog; Mammary gland neoplasms; TLRs; Real-time PCR
TLRs are important PRRs in innate immunity that are expressed on immune cells and mammary epithelial cells. This study aimed to investigate the correlation between histologic types and grades of neoplasms and TLRs gene expression levels. PCR analysis showed overexpression of TLR 3, 4, and 9 mRNA, with different tumor types and grades demonstrating varying expression levels. However, the correlation between histopathological characteristics and TLRs mRNA expression level was insignificant.
TLRs are a class of PRRs that play a vital role in innate immunity. TLRs are expressed on immune cells and mammary epithelial cells. They can promote tumor growth, angiogenesis, invasion, and viability signaling. The current study aimed to test the correlation between histologic types and grades of neoplasms and TLRs gene expression levels. Twenty-one tissue samples of canine mammary neoplasms were stained with H&E. Then, it evaluated histologic type and grade according to the methods of Goldschmidt et al. and Pen similar to a, respectively. We established real-time PCR quantification assays to measure the mRNA abundances of TLRs in normal and neoplastic mammary glands. Profile pattern of TLR 1, 2, 3, 4, 5, 6, and 9 genes expression in canine mammary glands performed in 21 samples of mammary gland neoplasms and three non-neoplastic mammary gland samples from normal dogs. TLR 3, 4, and 9 mRNA overexpression were detected. In addition, tubulopapillary carcinoma grade II, SCC grade III, and carcinoma mixed type grade II demonstrated the highest relative TLR-3, and 9 mRNA expression levels. Complex carcinoma grade I, ductal carcinoma grade II, and anaplastic carcinoma grade II showed the highest relative TLR4 mRNA expression level. Although histopathological characteristics of tumors, including histologic type, grade, and inflammation, influenced TLRs mRNA expression level, such correlation was insignificant (P > 0.05).
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