Ion current rectification-nanopipette technique for single-cell analysis

Article Chemistry, Multidisciplinary

An Integrated Dual-Functional Nanotool Capable of Studying Single-Cell Epigenetics and Programmable Gene Regulation

Xiao-Mei Shi et al.

Summary: Study investigates the dual functionality of using a DNAzyme confined within a nanopipette to study single-cell epigenetics and programmable gene regulation.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023)

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Article Chemistry, Multidisciplinary

Electrochemical Single-Cell Protein Therapeutics Using a Double-Barrel Nanopipette

Xiao-Mei Shi et al.

Summary: Single-cell protein therapeutics has the potential to enhance our understanding of how a specific protein treats cells individually instead of averaging across populations. A double-barrel nanopipette was used in this study to address the challenge. The results showed that delivering protein DJ-1 into cells can upregulate antioxidant protein and protect neural cells from oxidative stress. This nanotool provides a perspective for future single-cell therapies involving different therapeutic modalities.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023)

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Review Oncology

Single-cell profiling of tumour evolution in multiple myeloma - opportunities for precision medicine

Ankit K. Dutta et al.

Summary: This Review discusses the application of single-cell studies in multiple myeloma and its precursor stages, revealing the genetic heterogeneity and clonal evolution patterns. It provides guidance for early diagnosis, risk stratification, and treatment strategies.

NATURE REVIEWS CLINICAL ONCOLOGY (2022)

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Article Multidisciplinary Sciences

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states

Hanbing Song et al.

Summary: The study characterized the microenvironment response to prostate cancer using single-cell RNA-seq and organoids, identifying tumor-associated epithelial cell states and club cells.

NATURE COMMUNICATIONS (2022)

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Article Chemistry, Multidisciplinary

Spatiotemporally Resolved Protein Detection in Live Cells Using Nanopore Biosensors

Hongshuai Zhang et al.

Summary: This study developed a dual-nanopore biosensor for label-free and spatiotemporal monitoring of proteins in living cells. By using affinity-tunable peptide probes, the sensor demonstrated reversible and sensitive detection of protein kinase A (PKA) catalytic subunit, with the ability to effectively eliminate interferences from cell membrane resistance and coexisting species. The sensor was also applied successfully in monitoring dynamic PKA activity changes under different stimulations in single live cells.

ACS NANO (2022)

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Article Chemistry, Multidisciplinary

Chemical Measurement and Analysis: from Phenomenon to Essence

Wei Zhao et al.

Summary: This article summarizes the achievements of the research group in optical, electrochemical, and mass spectrometry analysis and imaging. They have made significant contributions in plasmonic imaging, electrogenerated chemiluminescence analysis, and nanopipette-based electrochemical analysis. Their research works have advanced the development of chemical measurement and analysis.

CHINESE JOURNAL OF CHEMISTRY (2022)

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Article Oncology

Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level

Yingcheng Wu et al.

Summary: This study presents a single-cell and spatial atlas of colorectal liver metastasis, revealing highly metabolically activated MRCP+ CCL18(+) M2-like macrophages at the metastatic sites. Effective neoadjuvant chemotherapy can slow down this metabolic activation, raising the possibility of targeting metabolic pathways in metastasis.

CANCER DISCOVERY (2022)

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Article Chemistry, Multidisciplinary

Organic Molecular Probe Enabled Ionic Current Rectification toward Subcellular Detection of Glutathione with High Selectivity, Sensitivity, and Recyclability

Yi-Li Liu et al.

Summary: Single-cell interrogation using solid-state nanoprobes allows for understanding the connection between cellular behavior and heterogeneity. A generic ionic current rectification (ICR) single-cell methodology is developed based on the charge property of the organic molecular probe (OMP), enabling subcellular detection of glutathione (GSH) with high selectivity, sensitivity, and recyclability.

ACS SENSORS (2022)

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Article Chemistry, Multidisciplinary

Translocation of Specific DNA Nanocarrier through an Ultrasmall Nanopipette: Toward Single-Protein-Molecule Detection with Superior Signal-to-Noise Ratio

Xian Zhang et al.

Summary: The use of functional DNA nanostructures as carriers to transport proteins through solid-state nanopores has shown significant progress in single-protein-molecule detection. In this study, a 6.8 nm DNA tetrahedron was used as a carrier to successfully detect acetylcholinesterase with high specificity, low detection limit, and superior signal-to-noise ratio through an ultrasmall nanopipet. The practical applicability of this method was validated using serum samples from patients with Alzheimer's disease.

ACS NANO (2022)

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Article Chemistry, Analytical

Glass Nanopore Detection of Copper Ions in Single Cells Based on Click Chemistry

Ping Hu et al.

Summary: In this study, a sensing platform based on functionalized nanopores was developed to monitor the change of intracellular copper ions, providing insights into redox balance and events in cells. The platform utilized a copper-catalyzed click reaction to modify the rectification behavior of the nanopores, showing a good response to intracellular copper ion concentration.

ANALYTICAL CHEMISTRY (2022)

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Article Chemistry, Analytical

Double-Barreled Micropipette Enables Neuron-Compatible In Vivo Analysis

Yifei Pan et al.

Summary: Iontronic sensors based on ion transport have been used for in vivo neurochemical assay, but the interference on spontaneous neuronal activity has not been addressed. This study used finite element simulation to quantify the electric field distribution of a double-barreled micropipette, and found parameters such as pore size and applied voltage that balanced sensitivity and interference. The double-barreled micropipette is a pH sensor that has successfully been applied to real-time pH sensing in rat brain.

ANALYTICAL CHEMISTRY (2022)

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Article Chemistry, Analytical

A highly sensitive and selective artificial nanochannel for in situ detection of hydroxyl radicals in single living cell

Feifeng Yang et al.

Summary: This study presents a novel glass nanopore regulated by hydroxyl radicals, with excellent selective response and efficient regulation of ion transportation. It provides insight into the role of hydroxyl radicals in pathological events and shows promising potential for biomedical research.

ANALYTICA CHIMICA ACTA (2022)

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Article Chemistry, Analytical

Development of Dual-Nanopore Biosensors for Detection of Intracellular Dopamine and Dopamine Efflux from Single PC12 Cell

Tao Zhao et al.

Summary: This study presents a dual-nanopore biosensor for the detection of dopamine at the single-cell level. The biosensor allows rapid and specific detection of dopamine, as well as monitoring changes in dopamine concentration within individual cells.

ANALYTICAL CHEMISTRY (2022)

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Article Chemistry, Multidisciplinary

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

Yi-Fan Ruan et al.

Summary: A dual-functional photocathodic single-cell nanotool has been developed for direct intracellular drug delivery and evaluation of oxidative stress, pushing the boundary of current single-cell electroanalysis.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

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Article Chemistry, Analytical

Target-Triggered Assembly in a Nanopipette for Electrochemical Single-Cell Analysis

Yi-Fan Ruan et al.

Summary: Engineered nanopipette tools have been shown to be a powerful approach for electrochemical nanosensing, with a generic method of target-triggered assembly of aptamers in a nanopipette for nanosensing demonstrated here. The method allows for sensitive and rapid electrochemical single-cell analysis of adenosine triphosphate (ATP), a ubiquitous energy source in life and important signaling molecules in many physiological processes.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Glutathione Content Detection of Single Cells under Ingested Doxorubicin by Functionalized Glass Nanopores

Ping Hu et al.

Summary: In this study, a novel cG-nanopore was developed for the specific detection of intracellular GSH in single cells. The method showed good responsiveness and selectivity, successfully quantifying the changes in GSH content in single cells incubated with drugs.

ANALYTICAL CHEMISTRY (2021)

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Article Biochemical Research Methods

Single-cell chromatin state analysis with Signac

Tim Stuart et al.

Summary: Signac is a comprehensive toolkit for the analysis of single-cell chromatin data, enabling end-to-end analysis and interoperability with the Seurat package for multimodal analysis.

NATURE METHODS (2021)

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Article Chemistry, Multidisciplinary

Photocontrolled Nanopipette Biosensor for ATP Gradient Electroanalysis of Single Living Cells

Si-Yuan Yu et al.

Summary: The study introduces a photocontrolled nanopipette capable of probing cellular ATP gradient at single-cell level with high sensitivity, selectivity, and reversibility, utilizing ATP-specific azobenzene-incorporated DNA aptamer strands for sensible transduction of variable nanopore size by ionic currents. The photoisomerized conformational change ensures noninvasive regeneration and repeated detection, allowing inducement and inhibition of cellular ATP to be probed.

ACS SENSORS (2021)

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Article Biochemical Research Methods

The emerging landscape of single-molecule protein sequencing technologies

Javier Antonio Alfaro et al.

Summary: Single-cell profiling methods have greatly impacted the understanding of cellular heterogeneity, although proteome profiling at the single-cell level is still in the early stages. The development of new technologies, such as single-molecule protein sequencing and innovations in mass spectrometry, will eventually enable broad sequence coverage in single-cell proteomics, leading to new biological discoveries and opportunities for ultra-sensitive disease diagnostics.

NATURE METHODS (2021)

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Review Chemistry, Analytical

Electrochemical Sensing at a Confined Space

Si-Min Lu et al.

ANALYTICAL CHEMISTRY (2020)

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Article Chemistry, Multidisciplinary

Real-time and in-situ intracellular ATP assay with polyimidazolium brush-modified nanopipette

Kailin Zhang et al.

SCIENCE CHINA-CHEMISTRY (2020)

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Review Chemistry, Multidisciplinary

Bioanalysis in single cells: current advances and challenges

Hong-Yuan Chen

SCIENCE CHINA-CHEMISTRY (2020)

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Article Chemistry, Multidisciplinary

The double life of conductive nanopipette: a nanopore and an electrochemical nanosensor

Rui Jia et al.

CHEMICAL SCIENCE (2020)

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Article Chemistry, Multidisciplinary

In situ detection of hydroxyl radicals in mitochondrial oxidative stress with a nanopipette electrode

Wenting Chen et al.

CHEMICAL COMMUNICATIONS (2020)

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Review Electrochemistry

Review-Recent Advances in Nanosensors Built with Pre-Pulled Glass Nanopipettes and Their Applications in Chemical and Biological Sensing

Megan Chang et al.

JOURNAL OF THE ELECTROCHEMICAL SOCIETY (2020)

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Article Chemistry, Multidisciplinary

Glucose level determination in single cells in their satiety and starvation states using an enzymatic functional glass nanopore

Dandan Wang et al.

CHEMICAL COMMUNICATIONS (2020)

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Article Chemistry, Multidisciplinary

Single-cell ATP detection and content analyses in electrostimulus-induced apoptosis using functionalized glass nanopipettes

Dandan Wang et al.

CHEMICAL COMMUNICATIONS (2020)

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Review Chemistry, Multidisciplinary

Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells

Ru-Jia Yu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

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Article Chemistry, Multidisciplinary

Biomolecule-Functionalized Solid-State Ion Nanochannels/Nanopores: Features and Techniques

Defang Ding et al.

SMALL (2019)

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Review Chemistry, Analytical

Confined Nanopipette-A new microfluidic approach for single cell analysis

Si-Min Lu et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Multidisciplinary

Nanostars on Nanopipette Tips: A Raman Probe for Quantifying Oxygen Levels in Hypoxic Single Cells and Tumours

Thanh Danh Nguyen et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

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Article Chemistry, Analytical

Fabrication and Use of Nanopipettes in Chemical Analysis

Shudong Zhang et al.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 11 (2018)

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Review Chemistry, Multidisciplinary

Bioinspired smart asymmetric nanochannel membranes

Zhen Zhang et al.

CHEMICAL SOCIETY REVIEWS (2018)

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Article Chemistry, Multidisciplinary

Functionalized Quartz Nanopipette for Intracellular Superoxide Sensing: A Tool for Monitoring Reactive Oxygen Species Levels in Single Living Cell

Rifat Emrah Ozel et al.

ACS SENSORS (2018)

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Article Chemistry, Multidisciplinary

Ultrasmall Nanopipette: Toward Continuous Monitoring of Redox Metabolism at Subcellular Level

Juan Song et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

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Article Chemistry, Analytical

Ultrafast Mapping of Subcellular Domains via Nanopipette-Based Electroosmotically Modulated Delivery into a Single Living Cell

Ruo-Can Qian et al.

ANALYTICAL CHEMISTRY (2018)

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Article Chemistry, Multidisciplinary

Electron-Transfer Gated Ion Transport in Carbon Nanopipets

Dengchao Wang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

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Editorial Material Chemistry, Multidisciplinary

Smart Gating Multi-Scale Pore/Channel-Based Membranes

Xu Hou

ADVANCED MATERIALS (2016)

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Article Chemistry, Multidisciplinary

Single Cell Glucose Nanosensor Verifies Elevated Glucose Levels in Individual Cancer Cells

Raphael A. S. Nascimento et al.

NANO LETTERS (2016)

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Review Chemistry, Multidisciplinary

Fundamental studies and practical applications of bio-inspired smart solid-state nanopores and nanochannels

Huacheng Zhang et al.

NANO TODAY (2016)

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Review Chemistry, Multidisciplinary

Biomimetic Solid-State Nanochannels: From Fundamental Research to Practical Applications

Kai Xiao et al.

SMALL (2016)

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Article Chemistry, Multidisciplinary

Single-cell intracellular nano-pH probes

Rifat Emrah Oezel et al.

RSC ADVANCES (2015)

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Article Chemistry, Multidisciplinary

From symmetric to asymmetric design of bio-inspired smart single nanochannels

Huacheng Zhang et al.

CHEMICAL COMMUNICATIONS (2013)

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Article Chemistry, Physical

Ion current rectification inversion in conic nanopores: Nonequilibrium ion transport biased by ion selectivity and spatial asymmetry

Yu Yan et al.

JOURNAL OF CHEMICAL PHYSICS (2013)

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Review Chemistry, Multidisciplinary

Biomimetic smart nanopores and nanochannels

Xu Hou et al.

CHEMICAL SOCIETY REVIEWS (2011)

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Review Chemistry, Analytical

Applications of nanopipettes in the analytical sciences

Celeste A. Morris et al.

ANALYST (2010)

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Review Chemistry, Multidisciplinary

Engineered voltage-responsive nanopores

Zuzanna S. Siwy et al.

CHEMICAL SOCIETY REVIEWS (2010)

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Article Chemistry, Multidisciplinary

Single Conical Nanopores Displaying pH-Tunable Rectifying Characteristics. Manipulating Ionic Transport With Zwitterionic Polymer Brushes

Basit Yameen et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

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Ion current rectification-nanopipette technique for single-cell analysis

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