期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 46, 期 4, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2023.126438
关键词
Fontisphaera; Fontisphaeraceae; Limisphaerales; Thermophilic; Hydrolytic; Baikal lake
A novel bacterium, strain B-154 T, was isolated from a terrestrial hot spring in the Baikal lake region. This strain is facultatively anaerobic and moderately thermophilic, with a growth temperature range of 30-57°C and pH range of 5.1-8.4. It is a chemoorganoheterotroph, capable of utilizing various mono-, di-, and polysaccharides. Phylogenomic analysis revealed that strain B-154 T belongs to a novel genus and species within the Verrucomicrobiota.
A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 T, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30-57 & DEG;C and within a pH range of 5.1-8.4 with the optimum at 50 & DEG;C and pH 6.8-7.1. Strain B-154 T was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, galactan, galactomannan, glucomannan, xyloglucan, pullulan, arabinan, lichenan, beta-glucan, pachyman, locust bean gum, xanthan gum). It did not require sodium chloride or yeast extract for growth. Major cellular fatty acids were anteiso-C15:0, iso-C16:0 and iso-C14:0. The respiratory quinone was MK-7. The complete genome of strain B-154 T was 4.73 Mbp in size; its G + C content was 61%. According to the phylogenomic analysis strain B-154 T forms a separate family-level phylogenetic lineage. Moreover, together with Limisphaera ngatamarikiensis and Pedosphaera parvula this strain forms a separate order-level phylogenetic lineage within Verrucomicrobiae class. Hence, we propose a novel order, Limisphaerales ord. nov., with two families Limisphaeraceae fam. nov. and Fontisphaeraceae fam. nov., and a novel genus and species Fontisphaera persica gen. nov., sp. nov. with type strain B-154 T. Ecogenomic analysis showed that representatives of the Limisphaerales are widespread in various environments. Although some of them were detected in hot springs the majority of Limisphaerales (54% of the studied metagenomeassembled genomes) were found in marine habitats. This study allowed a better understanding of physiology and ecology of Verrucomicrobiota - a rather understudied bacterial phylum.
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