Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.

Automated summary

In this study, we generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line, riPSC05, through induced reprogramming of fibroblasts using non-integrating Sendai virus technology. The authenticity of riPSC05 was confirmed through various evaluations, including the expression of pluripotent and self-renewal markers, directed differentiation towards three germ layers, karyotyping, and STR analysis.