期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 296, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2023.122658
关键词
Nile red; Fluorescent probe; H2Sn; Cell imaging; Zebrafish imaging
类别
To achieve the visual monitoring of H2Sn levels in vivo, a series of fluorescent probes NR-BS were constructed, among which probe NR-BS4 was optimized due to its wide linear range and low interference from biothiols. NR-BS4 also exhibited a broad pH tolerance range and high sensitivity. The intracellular imaging studies indicated that NR-BS4 can be successfully used to monitor the levels of exogenous and endogenous H2Sn in vivo.
Hydrogen polysulfide (H2Sn, n > 1) has a valuable function in various aspects of biological regulation. Therefore, it is of great significance to realize the visual monitoring of H2Sn levels in vivo. Herein, a series of fluorescent probes NR-BS were constructed by changing types and positions of substituents on the benzene ring of benzenesulfonyl. Among them, probe NR-BS4 was optimized due to its wide linear range (0 similar to 350 mu M) and little interference from biothiols. In addition, NR-BS4 has a broad pH tolerance range (pH = 4 similar to 10) and high sensitivity (0.140 mu M). In addition, the PET mechanism of probe NR-BS4 and H2Sn was demonstrated by DFT calculations and LC-MS. The intracellular imaging studies indicate that NR-BS4 can be successfully devoted to monitor the levels of exogenous and endogenous H2Sn in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据