Phenolic constituents of six Hypericum species from Turkiye and their chemotaxonomic relevance

Hypericum species have been of great interest to mankind for centuries and Tueurorkiye is a global intersection for these species. In the present study, 20 wild populations of six under-exploited species of Turkish Hyperi-cum from four sections (Hypericaceae; H. orientale, H. bupleuroides, H. venustum, H. hirsutum, H. linarioides and H. androsaemum) were analyzed qualitatively and quantitatively for the presence of 16 phenolic com-pounds by detailed high-performance liquid chromatography (HPLC) analyses for the first time. Except for quercetin, which was not accumulated by H. bupleuroides, H. venustum and H. linarioides, and hyperoside which was only accumulated by H. bupleuroides and H. venustum, all phenolics were detected at different levels depending on species and their populations. In order to differentiate the samples according to their geo-graphical origin and botanical section, as well as to elucidate the function of the phenolics as chemotaxonomic markers in taxonomy of the Hypericum genus, two multivariate approaches namely, hierar-chical cluster analysis (HCA) and principal component analysis (PCA) were performed. The results indicate a significant inter and intra species chemovariability, which has little chemosystemic significance for the taxa studied and makes a poor contribution to the chemotaxonomic understanding of the Turkish Hypericum spe-cies. The presented data could be possibly beneficial in clarifying evolutionary relationships within the botanical sections of the genus Hypericum and offer interesting proposals for further studies in the chemotax-onomy and pharmacognosy of this genus.& COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.

Automated summary

In this study, the qualitative and quantitative analysis of 16 phenolic compounds in under-exploited Turkish Hypericum species was conducted using HPLC for the first time. The results showed variations in compound levels depending on species and populations. Hierarchical cluster analysis and principal component analysis were used to differentiate samples based on geographical origin and botanical classification. The findings provide valuable data for clarifying evolutionary relationships and further research in chemotaxonomy and pharmacognosy of the Hypericum genus.