期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 393, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.134328
关键词
Fluorescent probe; Mitochondrial fragmentation; Morphological changes; Leakage-free
Mitochondrial fragmentation is a crucial physiological process associated with energy production, metabolism, and apoptosis. However, the details of morphological changes during this process are rarely reported. In this study, a leakage-free fluorescent probe called IP-8 was developed and successfully used to visualize and track the details of mitochondrial fragmentation process in real-time and in situ. IP-8 was suggested as a useful tool for studying mitochondrial morphology changes.
Mitochondrial fragmentation is a crucial physiologic process that is closely associated with energy production, metabolism, and apoptosis. However, the details of morphological changes are rarely reported. To visualize them, a leakage-free fluorescent probe composed of an indole-pyridinium and a C8-alkyl chain, IP-8, was constructed. IP-8 could firmly immobilize in mitochondria by strong hydrophobic interaction between the C8-alkyl chain and phospholipid bilayer of the mitochondrial inner membrane, thus realizing the long-term staining without leakage regardless of mitochondrial membrane potential (MMP) changes. Importantly, using IP-8, three details during mitochondrial fragmentation process, from granule to donut, eventually formed cluster, were successfully visualized real-time and in situ. Therefore, IP-8 was suggested as a useful tool for visualizing and tracking of mitochondrial morphology changes.
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