期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 386, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.133741
关键词
Nanozymes; Chitosan; Platinum nanostructures; Dual -readout; Photothermal; Colorimetric; Cholesterol
A nanozyme-mediated dual-readout (colorimetric and photo-thermal) bioassay was developed for straightforward and sensitive detection of cholesterol. The dual-readout sensor combines cholesterol oxidase-catalyzed cholesterol oxidation with CS@Pt NS-catalyzed TMB oxidation, using oxidized TMB as a probe for colorimetric and photothermal readouts. The linear detection range is 0-2 mM, with a detection limit of 45 μM for the colorimetric assay and 25 μM for the photothermal assay. The sensor successfully detects cholesterol in spiked human serum with acceptable accuracy.
Point-of-care testing has been extensively utilized for in-home diagnosis, customized testing, and pandemic monitoring. The current study aims to develop a nanozyme-mediated dual-readout (colorimetric and photo -thermal) bioassay for straightforward and sensitive detection of cholesterol. The nanozyme, cauliflower-like platinum nanostructures (CS@Pt NS) were synthesized by chemically reducing the Pt precursor in the chito-san (CS) solution. CS as a stabilizer excels at controlling the surface properties, enhancing peroxidase-like ac-tivity, and improving colloidal dispersion, and stability. The dual-readout sensor for cholesterol detection was proposed by combining cholesterol oxidase-catalyzed cholesterol oxidation with CS@Pt NS-catalyzed TMB oxidation. The oxidized TMB (TMBox) acts as a probe for both colorimetric and photothermal readouts because of having excellent absorbance in visible and near-infrared (NIR) regions. The absorption of NIR laser-driven light by the TMBox in the reaction solution generates heat, which is proportional to the concentration of cholesterol. The dual-readout sensor shows a linear detection range of 0-2 mM, with a limit of detection (LOD) of 45 mu M for the colorimetric assay and 25 mu M for the photothermal assay. Furthermore, the sensor successfully detects cholesterol in spiked human serum with acceptable accuracy.
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