4.7 Article

The cysteine-induced BiOBr as a novel photoactive material with high photoelectric efficiency for ultrasensitive detection of microRNA

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SENSORS AND ACTUATORS B-CHEMICAL
卷 387, 期 -, 页码 -

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ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.133770

关键词

Photoelectrochemical biosensor; BiOBr; S -doping; Oxygen vacancy; MicroRNA

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In this study, a novel photoactive material, cysteine-induced BiOBr (cys-BiOBr), was prepared to construct a highly efficient photochemical (PEC) biosensor for ultrasensitive detection of microRNA-155 (miRNA-155). The photoelectric efficiency of cys-BiOBr showed a 6-fold increase compared to individual BiOBr due to the doping of S, which narrowed the band gap, enhanced light absorption and carrier separation, and increased the surface oxygen vacancy concentration to prevent recombination of electron-hole pairs. A few miRNA-155 molecules were amplified through an exponential amplification reaction (EXPAR) to form abundant steady G-quadruplexes, which immobilized hemin and catalyzed H2O2 decomposition to significantly amplify the photocurrent signal. The proposed PEC biosensor exhibited a wide detection range from 0.5 fM to 1 nM with a detection limit of 0.13 fM, providing a new method for sensitive detection of biomolecules and showing great potential in early clinical diagnosis.
In this work, cysteine-induced BiOBr (cys-BiOBr) was prepared as a novel photoactive material with high photoelectric efficiency to construct a photochemical (PEC) biosensor for ultrasensitive detection of microRNA-155 (miRNA-155). Impressively, the photocurrent signal of cys-BiOBr showed an increase of 6 times compared to that of individual BiOBr, since the doping of S not only narrowed the band gap to enhance light absorption and accelerate carrier separation, but also augmented the surface oxygen vacancy concentration for preventing electron-hole pairs recombination, resulting in the obvious improvement of photoelectric efficiency. Furthermore, through exponential amplification reaction (EXPAR), a few miRNA-155 could be derived into abundant steady G-quadruplexes for immobilizing hemin to form the G-quadruplex/hemin complex for catalyzing H2O2 decomposition to significantly amplify photocurrent signal. The proposed PEC biosensor performed a wide range from 0.5 fM to 1 nM with a detection limit of 0.13 fM. This strategy provided a new method to prepare the highly efficient photoactive material for sensitive detection of biomolecules and exhibited enormous potential in early clinical diagnosis.

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