期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 392, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2023.134081
关键词
Non-enzymatic probe; Myocardial infarction; Cardiac biomarker; Glycosaminoglycan; Heparin; Biosensor; Cardiovascular disease
In this study, positively charged green-emitting gold nanoclusters were synthesized and conjugated with heparin to serve as a fluorescence probe for the non-enzymatic detection of cardiac troponin I.
Despite the very high specificity and simplicity, antibody-based assays require high costs and top-end preservation facilities to develop an efficient biosensor. We constructed An antibody-free fluorescence probe for cardiac troponin -I, the 'gold standard' marker for diagnosing myocardial infarction. In the present work, positively charged green-emitting gold nanoclusters were synthesized through a one-pot microwave-mediated method, and the fluorescence response with heparin was studied. These heparin-conjugated gold nanoclusters were further utilized as a fluorescence probe for the non-enzymatic detection of cardiac troponin I. The linear detection of troponin with a low limit of detection of 0.1631 ng/mL was achieved with the probe. The challenging interfering perturbation of the co-existing biomolecules was evaluated, among which only glutathione showed an insignificant turn-on response within the biological limit.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据