4.7 Article

A centrifugal microfluidic platform for rapid detection of pathogens based on chitosan nucleic acid extraction and RAA-T7-CRISPR/Cas13a nucleic acid detection

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Chemistry, Analytical

Total integrated centrifugal genetic analyzer for point-of-care Covid-19 testing with automatic and high-throughput capability

Hau Van Nguyen et al.

Summary: The proposed prototype portable genetic analyzer can perform RNA extraction, RT-LAMP amplification, and fluorescence detection for ten samples simultaneously in an automatic and high-throughput manner. It utilizes zigzag aliquoting channels and passive valves for sample partitioning and extraction operations.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Chemistry, Analytical

Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood

Nan Zong et al.

Summary: A centrifugal microfluidic platform was developed for combined pretreatment and molecular diagnosis. Multiple signal enhancement systems were used to avoid false-negative results. The platform successfully detected and genotyped hepatitis B virus from whole blood. Compared to traditional diagnostic processes, the platform significantly reduced time consumption and the need for professional labor.

ANALYTICAL CHEMISTRY (2022)

Article Environmental Sciences

Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring

Hamsa N. Gowda et al.

Summary: The study developed a portable pathogen analysis system that can detect bacteria in water within 1 hour, with the advantages of simple operation and high sensitivity.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

Article Chemistry, Analytical

A Salmonella serogroup rapid identification system for food safety based on high throughput microfluidic chip combined with recombinase aided amplification

Xinran Xiang et al.

Summary: A high throughput microfluidic system based on real-time recombinase aided amplification (RAA) was developed for simultaneous detection of Salmonella genus and five serogroups. The system showed advantages in detection time and result interpretation compared to conventional methods. It also exhibited excellent stabilities and accuracies in sample verification, indicating promising application prospects in pathogen assessment.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Review Chemistry, Analytical

Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing

Menna Mostafa et al.

Summary: This review provides a comprehensive overview of the current techniques developed for the detection of SARS-CoV-2, including detection methods in various body fluids and considerations of viral mutations. The detection methods are classified and discussed based on the measured biomarkers and measurement techniques, and the challenges and ways to overcome these challenges are addressed.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

Article Chemistry, Analytical

An electrochemical sensing method based on CRISPR/Cas12a system and hairpin DNA probe for rapid and sensitive detection of Salmonella Typhimurium

Yawen He et al.

Summary: In this study, an electrochemical sensing method was developed to detect S. Typhimurium using PCR and CRISPR / Cas12a technology. The method allows sensitive detection of the pathogen in pure cultures and spiked poultry meat.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Biochemical Research Methods

An automatic centrifugal system for rapid detection of bacteria based on immunomagnetic separation and recombinase aided amplification

Siyuan Wang et al.

Summary: This study presents an automated centrifugal system for rapid quantification of foodborne pathogenic bacteria. The system combines immunomagnetic separation for bacteria enrichment and recombinase aided amplification for nucleic acid detection. The system achieved efficient bacteria separation, nucleic acid extraction and fluorescent detection in a centrifugal disc, enabling Salmonella detection as low as 10 CFU mL(-1) in 1 hour.

LAB ON A CHIP (2022)

Article Fisheries

Application of the FTA elute card coupled with visual colorimetric loop-mediated isothermal amplification for the rapid diagnosis of Streptococcus agalactiae in farmed tilapia (Oreochromis niloticus)

Elodie Pepey et al.

Summary: The study tested the FTA-E/LAMP method for diagnosing Streptococcus agalactiae infections, showing high sensitivity and specificity in vitro, while requiring additional primers to differentiate other species. In vivo experiments on fish infection models demonstrated high accuracy and sensitivity.

JOURNAL OF FISH DISEASES (2021)

Article Biophysics

High-throughput human DNA purification on a centrifugal microfluidic device for rapid forensic sex-typing

Hau Van Nguyen et al.

Summary: The novel centrifugal disc is capable of purifying nucleic acid in a high-throughput manner, processing 30 forensic samples in a single run. It automatically distributes washing solution and elution buffer, featuring a superhydrophobic zigzag-shaped structure for efficient division. The entire process is operated by a portable workstation, enabling quick completion of genetic sex-typing.

BIOSENSORS & BIOELECTRONICS (2021)

Article Chemistry, Analytical

Multiplexed colorimetric detection of SARS-CoV-2 and other pathogens in wastewater on a 3D printed integrated microfluidic chip

Kun Yin et al.

Summary: The study developed a sensitive, multiplexed, colorimetric detection method for pathogen detection in wastewater samples, allowing for early warning of disease outbreaks. This method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip, enabling real-time analysis without complex equipment.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Biochemical Research Methods

A simple methodology for RNA isolation from bacteria by integration of formamide extraction and chitosan-modified silica purification

Xiaoli Zhao et al.

Summary: Researchers have developed a rapid and cost-effective method for isolating RNA from bacteria, which can be completed within 15 minutes and yields RNA with comparable purity to that of commercial kits. The extracted RNA has been successfully used in downstream enzymatic reactions, showing promise for gene expression analyses in bacterial organisms.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2021)

Article Engineering, Biomedical

A centrifugal microfluidic emulsifier integrated with oil storage structures for robust digital LAMP

Huo Peng et al.

BIOMEDICAL MICRODEVICES (2020)

Article Food Science & Technology

Development of a Capture ELISA for Rapid Detection of Salmonella enterica in Food Samples

Tiziana Di Febo et al.

FOOD ANALYTICAL METHODS (2019)

Article Biochemical Research Methods

SHERLOCK: nucleic acid detection with CRISPR nucleases

Max J. Kellner et al.

NATURE PROTOCOLS (2019)

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

Article Biochemical Research Methods

A chitosan coated monolith for nucleic acid capture in a thermoplastic microfluidic chip

Eric L. Kendall et al.

BIOMICROFLUIDICS (2014)

Article Biochemical Research Methods

A rotary microsystem for simple, rapid and automatic RNA purification

Byung Hyun Park et al.

LAB ON A CHIP (2012)

Article Chemistry, Analytical

Chitosan as a polymer for pH-induced DNA capture in a totally aqueous system

Weidong Cao et al.

ANALYTICAL CHEMISTRY (2006)