期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 897, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2023.165348
关键词
Gut dysbiosis; MMTV-Erbb2 mice; Breast cancer; Cadmium; Glutamine metabolism
Cadmium (Cd) exposure has been reported to be linked to breast cancer (BC) development. In this study, a transgenic mouse model (MMTV-Erbb2) was used to investigate the effects of Cd exposure on BC tumorigenesis. The results showed that Cd exposure accelerated tumor growth and increased Ki67 density in MMTV-Erbb2 mice. Furthermore, Cd exposure disturbed gut microbiota homeostasis, altering Glutamine (Gln) metabolism and promoting mammary tumorigenesis. This study provides new insights into Cd exposure-mediated carcinogenesis.
Cadmium (Cd) isa heavy metal that has been widely reported to be linked to the onset and progression of breast cancer (BC). However, the mechanism of Cd-induced mammary tumorigenesis remains elusive. In our study, a transgenic mouse model that spontaneously develops tumors through overexpression of wild-type Erbb2 (MMTV-Erbb2) was constructed to investigate the effects of Cd exposure on BC tumorigenesis. The results showed that oral exposure to 3.6 mg/L Cd for 23 weeks dramatically accelerated tumor appearance and growth, increased Ki67 density and enhanced focal necrosis and neovascularization in the tumor tissue of MMTV-Erbb2 mice. Notably, Cd exposure enhanced glutamine (Gln) metabolism in tumor tissue, and 6-diazo-5-oxo-L-norleucine (DON), a Gln metabolism antagonist, inhibited Cd-induced breast carcinogenesis. Then our metagenomic sequencing and mass spectrometrybased metabolomics confirmed that Cd exposure disturbed gut microbiota homeostasis, especially Helicobacter and Campylobacter abundance remodeling, which altered the gut metabolic homeostasis of Gln. Moreover, intratumoralGln metabolism profoundly increased under Cd-elevated gut permeability. Importantly, depletion of microbiota with an antibiotic cocktail (AbX) treatment led to a significant delay in the appearance of palpable tumors, inhibition of tumor growth, decrease in tumor weight, reduction in Ki6 7 expression and low-grade pathology in Cd-exposed MMTV-Erbb2 mice. Also, transplantation of Cd-modulated microbiota decreased tumor latency, accelerated tumor growth, increased tumor weight, upregulated Ki67 expression and exacerbated neovascularization as well as focal ne-crosis in MMTV-Erbb2 mice. In summary, Cd exposure induced gut microbiota dysbiosis, elevated gut permeability and increased intratumoral Gln metabolism, leading to the promotion of mammary tumorigenesis. This study provides novel insights into environmental Cd exposure-mediated carcinogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据