期刊
SCIENCE
卷 382, 期 6666, 页码 92-98出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.adg7921
关键词
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There is a difference in accessory chlorophyll between marine and terrestrial photosynthesis. We identified the CHLC dioxygenase from the marine diatom as the Chl c synthase, answering a long-standing question in the field.
Marine and terrestrial photosynthesis exhibit a schism in the accessory chlorophyll (Chl) that complements the function of Chl a: Chl b for green plants versus Chl c for most eukaryotic phytoplankton. The enzymes that mediate Chl c biosynthesis have long remained elusive. In this work, we identified the CHLC dioxygenase (Phatr3_J43737) from the marine diatom Phaeodactylum tricornutum as the Chl c synthase. The chlc mutants lacked Chl c, instead accumulating its precursors, and exhibited growth defects. In vitro, recombinant CHLC protein converted these precursors into Chl c, thereby confirming its identity. Phylogenetic evidence demonstrates conserved use of CHLC across phyla but also the existence of distinct Chl c synthases in different algal groups. Our study addresses a long-outstanding question with implications for both contemporary and ancient marine photosynthesis.
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