期刊
MONATSHEFTE FUR CHEMIE
卷 147, 期 6, 页码 1137-1142出版社
SPRINGER WIEN
DOI: 10.1007/s00706-016-1696-8
关键词
Acylation; Enzymes; Glycosides; Regioselectivity
资金
- Slovak Research and Development Agency [APVV-0846-12]
- Slovak Grant Agency for Science VEGA [2/0138/12]
- Research and Development Operational Programmes - ERDF (Centre of Excellence on Green Chemistry Methods and Processes, CEGreenI) [26240120001]
- Research and Development Operational Programmes - ERDF (Amplification of the Centre of Excellence on Green Chemistry Methods and Processes, CEGreenII) [26240120025]
Chromogenic substrate 4-nitrophenyl gallate was prepared in four steps and used for selection of hydrolases specific to gallic ester hydrolysis from among 22 commercial lipases, proteases, and crude glycanase cocktails. Enzymes displaying galloyl esterase activity were tested in regioselective galloylation of methyl beta-d-glucopyranoside with vinyl gallate. Lipozyme TL IM in acetonitrile was found to afford the highest conversion (37 %). The reaction proceeded with strict regioselectivity toward the primary hydroxyl of the glucopyranoside ring, giving in preparative scale 27 % of purified methyl 6-O-galloyl-beta-d-glucopyranoside as a sole product.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据