期刊
MOLECULES
卷 21, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/molecules21111577
关键词
enzymes; immobilization; stabilization; agar-agar; agarose; cross-linking; functionalization
资金
- Universita di Cagliari (Contributo di Ateneo per la Ricerca) [C.A.R. 2012-13-14, PRID 2015]
- MINECO from Spanish Government [CTQ2013-41507-R]
Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and sharply hydrophilic. These features-that could be further improved by means of covalent cross-linking-render them particularly suitable for enzyme immobilization with a wide range of derivatization methods taking advantage of chemical modification of a fraction of the polymer hydroxyls. The main properties of the polymer are described here, followed by a review of cross-linking and derivatization methods. Some recent, innovative procedures to optimize the catalytic activity and operational stability of the obtained preparations are also described, together with multi-enzyme immobilized systems and the main guidelines to exploit their performances.
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