期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 201, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2023.112365
关键词
Multi-omics; Postharvest vegetables; Preservation; Transcription; Translation
An integration profiling of transcriptome and metabolome was performed to understand the effects and molecular regulation mechanism of H2O2 on nucleic acid metabolism in yellowing and senescence process of postharvest broccoli. The results showed that H2O2 delayed yellowing and increased relative conductivity, as well as promoted DNA replication and repair, transcription and pre-mRNA processing, and translation process in broccoli. H2O2 also maintained higher contents of nucleic acid-related metabolites, DNA and RNA, ultimately delaying the senescence of postharvest broccoli.
To understand the effects and related molecular regulation mechanism of H2O2 on nucleic acid metabolism in yellowing and senescence process of postharvest broccoli, an integration profiling of transcriptome and metabolome was performed. Results showed that 2 mM H2O2 delayed the yellowing and increase of relative conductivity, whereas 3 mu M diphenylene iodonium (DPI, an inhibitor of H2O2 production) accelerated these processes center dot H2O2 treatment promoted DNA replication and repair by up-regulating replication protein A, DNA polymerase, and DNA ligase 1 expressions and increasing the essential building blocks of DNA (e.g., cytosine, guanosine 5' -diphosphate, cytidine, and adenosine) contents, thereby ensuring the integrity of genome in broccoli center dot H2O2 treatment promoted transcription and pre-mRNA processing by up-regulating the expressions of RNA polymerases and spliceosome-related genes, which promoted the biosynthesis of mature mRNA center dot H2O2 treatment also up-regulated PABPs expression, and thus to promote translation process in broccoli. Additionally, H2O2 promoted the recycle of mRNA and accumulation of adenosine, which was beneficial to timely change own gene profile in response to complex postharvest environmental changes and alleviate the energy deficiency suffered by postharvest broccoli. These results suggested that H2O2 is involved in the positive regulation of nucleic acid metabolism and maintained higher contents of nucleic acid-related metabolites, DNA and RNA, ultimately delaying the senescence of postharvest broccoli, which provided new insight for the regulatory mechanism of the yellowing and senescence in broccoli.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据