期刊
MOLECULES
卷 21, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/molecules21050549
关键词
phthalides; Angelicae Sinensis Radix; cytotoxicity activity; flow cytometry; neuroprotective effect
资金
- Program of International Science and Technology Cooperation of China [2011DFA32630]
- National Major Projects of Drug Discovery [2012ZX09103201-035]
- Program of Science and Technology of Shanxi Province [20140313008-14]
- Construction of the Science and Technology Basic Condition Platform of Shanxi Province [2014091022]
Seven phthalides, including a new dimeric one named tokinolide C (7), were isolated from Angelicae Sinensis Radix and characterized. The structures of these compounds were elucidated on the basis of comprehensive analysis of spectroscopic data and comparison with literature data. All of the compounds were evaluated for their cytotoxic activities against the A549, HCT-8, and HepG2 cancer cell lines. Riligustilide (4) showed cytotoxicity against three cancer cell lines, with IC50 values of 13.82, 6.79, and 7.92 mu M, respectively. Tokinolide A (6) and tokinolide C (6) exerted low cytotoxicity in these cancer cell lines, while the remaining compounds were inactive. Flow cytometry analysis was employed to evaluate the possible mechanism of cytotoxic action of riligustilide (4). We observed that compound 4 was able to arrest the cell cycle in the G1, S phases and induce apoptosis in a time-dependent manner in HCT-8 cell lines. In addition, these compounds were evaluated for neuroprotective effect against SH-SY5Y cells injured by glutamate. The result showed that ligustilide (1), Z-butylidenephthalide (3) and tokinolide A (6) exhibited significant neuroprotective effects.
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