Combined transcriptome and proteome analysis revealed the molecular regulation mechanisms of zinc homeostasis and antioxidant machinery in tobacco in response to different zinc supplies

Zinc (Zn) is an essential micronutrient for plants. Adequate regulation of Zn uptake, transport and distribution, and adaptation to Zn-deficiency stress or Zn-excess toxicity are crucial for plant growth and development. However, little has been done to understand the molecular responses of plants toward different Zn supply levels. In the present study, we investigated the growth and physiological responses of tobacco seedlings grown under Zn-completely deficient, Zn-limiting, Zn-normal, and Zn-4-fold sufficient conditions, respectively, and demonstrated that Zn deficiency/limitation caused oxidative stress and impaired growth of tobacco plants. Combined transcriptome and proteome analysis revealed up-regulation of genes/proteins associated with Zn uptake and distribution, including ZIPs, NAS3s, and HMA1s, and up-regulation of genes/proteins involved in regulation of oxidative stress, including SODs, APX1s, GPX6, and GSTs in tobacco seedlings in response to Zn deficiency/ limitation, suggesting that tobacco possessed mechanisms to regulate Zn homeostasis primarily through upregulation of the ZIPs-NAS3s module, and to alleviate Zn deficiency/limitation-induced oxidative stress through activation of the antioxidant machinery. Our results provide novel insights into the adaptive mechanisms of tobacco in response to different Zn supplies, and would lay a theoretical foundation for development of varieties of tobacco or its relatives with high tolerance to Zn-deficiency.

Automated summary

Zinc is essential for plant growth and development, and its regulation is crucial for adaptation to different zinc levels. This study investigates the responses of tobacco seedlings to zinc deficiency and limitation, revealing that oxidative stress and impaired growth occur under these conditions. Transcriptome and proteome analysis show the up-regulation of genes/proteins involved in zinc uptake, distribution, and antioxidative stress mechanisms, suggesting the existence of adaptive mechanisms in tobacco. These findings provide insights for the development of zinc-deficiency tolerant tobacco varieties.