期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 202, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2023.107969
关键词
Ailanthus prickly ash; Folklore plant; Repruning; Volatile organic compounds; Terpene synthase; Solid phase micro extraction
In this study, five candidate monoterpene synthase genes were cloned and characterized, and it was found that the leaves of Tana contain volatile terpenoids such as sabinene, geraniol, and (E)-& beta;-ocimene, which may contribute to the distinctive aroma of this plant. Real-time PCR analysis showed that two genes, Za_mTPS1 and Za_mTPS5, were expressed at higher levels in prickly leaves of cultivated Tana, suggesting their involvement in the unique aroma of this plant.
Tana (Zanthoxylum ailanthoides), a perennial deciduous species in the Rutaceae family, possesses leaves with a unique fragrance that indigenous peoples incorporate into their traditional cuisine. In Kalibuan, the cultivated tana trees were pruned repeatedly to maintain a shorter height, which led to the growth of new leaves that were spicier and pricklier. Tana leaves contain a range of volatile terpenoids, and the pungent aroma may arise from the presence of monoterpenoids. To gain insight into the biosynthetic pathway, five candidate monoterpene synthase genes were cloned and characterized using a purified recombinant protein assay. The main product of Za_mTPS1, Za_mTPS2, and Za_mTPS5 is sabinene, geraniol, and (E)-& beta;-ocimene, respectively. The main product of Za_mTPS3 and Za_mTPS4 is linalool. Real-time PCR analysis revealed that Za_mTPS1 and Za_mTPS5 are expressed at higher levels in prickly leaves of cultivated tana, suggesting that they may contribute to the distinctive aroma of this plant.
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