Chloroplast-targeted late embryogenesis abundant 1 increases alfalfa tolerance to drought and aluminum

Late embryogenesis-abundant (LEA) proteins are important stress-response proteins that participate in protecting plants against abiotic stresses. Here, we investigated LEA group 3 protein MsLEA1, containing the typically disordered and & alpha;-helix structure, via overexpression and RNA interference (RNAi) approaches in alfalfa (Medicago sativa L.) under drought and aluminum (Al) stresses. MsLEA1 was highly expressed in leaves and localized in chloroplasts. Overexpressing MsLEA1 increased alfalfa tolerance to drought and Al stresses, but downregulating MsLEA1 decreased the tolerance. We observed a larger stomatal aperture and a lower water use efficiency in MsLEA1 RNAi lines compared with wild-type plants under drought stress. Photosynthetic rate, Rubisco activity, and superoxide dismutase (SOD) activity increased or decreased in MsLEA1-OE or MsLEA1-RNAi lines, respectively, under drought and Al stress. Copper/zinc SOD (Cu/Zn-SOD), iron SOD (Fe-SOD), and Rubisco large subunit proteins (Ms1770) were identified as binding partners of MsLEA1, which protected chloroplast structure and function under drought and Al stress. These results indicate that MsLEA1 recruits and protects its target proteins (SOD and Ms1770) and increases alfalfa tolerance against drought and Al stresses. Chloroplast-targeted MsLEA1 increases the tolerance of alfalfa under drought and aluminum stresses via interacting with superoxide dismutase and Rubisco subunit protein.

Automated summary

The LEA group 3 protein MsLEA1 was investigated for its role in drought and aluminum stress tolerance in alfalfa. Overexpression of MsLEA1 increased tolerance, while downregulation decreased tolerance. MsLEA1 interacts with superoxide dismutase and Rubisco subunit protein to protect chloroplast structure and function, thereby increasing alfalfa's tolerance against drought and aluminum stresses.