Evolution of the spinach sex-linked region within a rarely recombining pericentromeric region

Sex chromosomes have evolved independently in many different plant lineages. Here, we describe reference genomes for spinach (Spinacia oleracea) X and Y haplotypes by sequencing homozygous XX females and YY males. The long arm of 185-Mb chromosome 4 carries a 13-Mb X-linked region (XLR) and 24.1-Mb Y-linked region (YLR), of which 10 Mb is Y specific. We describe evidence that this reflects insertions of autosomal sequences creating a Y duplication region or YDR whose presence probably directly reduces genetic recombination in the immediately flanking regions, although both the X and Y sex-linked regions are within a large pericentromeric region of chromosome 4 that recombines rarely in meiosis of both sexes. Sequence divergence estimates using synonymous sites indicate that YDR genes started diverging from their likely autosomal progenitors about 3 MYA, around the time when the flanking YLR stopped recombining with the XLR. These flanking regions have a higher density of repetitive sequences in the YY than the XX assembly and include slightly more pseudogenes compared with the XLR, and the YLR has lost about 11% of the ancestral genes, suggesting some degeneration. Insertion of a male-determining factor would have caused Y linkage across the entire pericentromeric region, creating physically small, highly recombining, terminal pseudoautosomal regions. These findings provide a broader understanding of the origin of sex chromosomes in spinach. The spinach X and Y haplotypes are obtained through de novo homozygous XX female and YY males, deciphering the origin and evolution of sex chromosome in Spinacia oleracea.

Automated summary

Reference genomes for the X and Y haplotypes of spinach (Spinacia oleracea) were described by sequencing homozygous XX females and YY males. The presence of a Y duplication region and differences in repetitive sequences and pseudogenes suggest degeneration and reduced recombination in the Y-linked region. These findings contribute to a broader understanding of the origin of sex chromosomes in spinach.