StMLP1, as a Kunitz trypsin inhibitor, enhances potato resistance and specifically expresses in vascular bundles during Ralstonia solanacearum infection

Miraculin-like proteins (MLPs), members of the Kunitz trypsin inhibitor (KTI) family that are present in various plants, have been discovered to have a role in defending plants against pathogens. In this study, we identified a gene StMLP1 in potato that belongs to the KTI family. We found that the expression of StMLP1 gradually increases during Ralstonia solanacearum (R. solanacearum) infection. We characterized the promoter of StMLP1 as an inducible promoter that can be triggered by R. solanacearum and as a tissue-specific promoter with specificity for vascular bundle expression. Our findings demonstrate that StMLP1 exhibits trypsin inhibitor activity, and that its signal peptide is essential for proper localization and function. Overexpression of StMLP1 in potato can enhance the resistance to R. solanacearum. Inhibiting the expression of StMLP1 during infection accelerated the infection by R. solanacearum to a certain extent. In addition, the RNA-seq results of the overexpression-StMLP1 lines indicated that StMLP1 was involved in potato immunity. All these findings in our study reveal that StMLP1 functions as a positive regulator that is induced and specifically expressed in vascular bundles in response to R. solanacearum infection.

Automated summary

Miraculin-like proteins (MLPs), belonging to the Kunitz trypsin inhibitor (KTI) family, have been found to play a role in defending plants against pathogens. In this study, a gene called StMLP1 was identified in potato, which showed increased expression during Ralstonia solanacearum infection. StMLP1 was characterized as an inducible promoter and specifically expressed in vascular bundles. Overexpression of StMLP1 enhanced resistance to R. solanacearum, while inhibiting its expression accelerated the infection. RNA-seq results further confirmed the involvement of StMLP1 in potato immunity.