期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1111/pbi.14162
关键词
polyhydroxybutyrate; polyhydroxyalkanoate; PHB; PHA; biopolymer; Camelina sativa
Plant-based production of polyhydroxyalkanoates (PHAs) and seed oil can provide a sustainable source of feedstocks for renewable fuels and plastics. This study successfully engineered Camelina sativa to produce PHA poly[(R)-3-hydroxybutyrate] (PHB) in the seed cytosol using a bacterial PHB biosynthetic pathway. By localizing the key enzymes to the cytosol and endoplasmic reticulum, stable polymer production was achieved with PHB levels reaching 10.2% of seed weight. This research represents a significant advancement towards commercial use of plant-based PHAs, connecting large-scale agricultural production with the plastics and renewable fuels markets.
Plant-based co-production of polyhydroxyalkanoates (PHAs) and seed oil has the potential to create a viable domestic source of feedstocks for renewable fuels and plastics. PHAs, a class of biodegradable polyesters, can replace conventional plastics in many applications while providing full degradation in all biologically active environments. Here we report the production of the PHA poly[(R)-3-hydroxybutyrate] (PHB) in the seed cytosol of the emerging bioenergy crop Camelina sativa engineered with a bacterial PHB biosynthetic pathway. Two approaches were used: cytosolic localization of all three enzymes of the PHB pathway in the seed, or localization of the first two enzymes of the pathway in the cytosol and anchoring of the third enzyme required for polymerization to the cytosolic face of the endoplasmic reticulum (ER). The ER-targeted approach was found to provide more stable polymer production with PHB levels up to 10.2% of the mature seed weight achieved in seeds with good viability. These results mark a significant step forward towards engineering lines for commercial use. Plant-based PHA production would enable a direct link between low-cost large-scale agricultural production of biodegradable polymers and seed oil with the global plastics and renewable fuels markets.
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