4.7 Article

Genome editing in rice using CRISPR/Cas12i3

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PLANT BIOTECHNOLOGY JOURNAL
卷 -, 期 -, 页码 -

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WILEY
DOI: 10.1111/pbi.14192

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CRISPR/Cas12i3; Multiplex DR-spacer Array; DNA structural variations; Base editors

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This study characterized the genome editing application of Cas12i3, a type V-I family endonuclease, in rice. A CRISPR/Cas12i3-based iMAGE system was developed, which efficiently edited various genes in rice and produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. Base editors using deactivated Cas12i3 were also developed, resulting in the generation of herbicide-resistant rice plants. These CRISPR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.
The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.

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