期刊
CHEMICAL ENGINEERING JOURNAL
卷 264, 期 -, 页码 735-743出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2014.12.011
关键词
Laminaria japonica; Cryptococcus curvatus; CSTR; Repeated batch; Fatty acid methyl esters (FAMEs); Biodiesel
资金
- National Research Foundation of South Korea (NRF) - Ministry of Education, Science and Technology South Korea [2011-0001108]
- Advanced Biomass R&D Center (ABC) of South Korea Grant - Ministry of Education, Science and Technology [ABC-2011-0028387]
- Marine Biotechnology Program - Ministry of Land, Transport and Maritime Affairs of South Korean Government, South Korea
- Ministry of Land, Transportation and Maritime Affairs (MLTM) of South Korean government
- National Research Foundation of South Korea - Ministry of Education, Science and Technology [R31-30005]
Marine biomass is a potential carbon resource for the production of bin-based energy and chemicals. Microbial lipids are competitive in producing biodiesel. In this study, Laminaria japonica (Saccharina japonica) was chosen as a model marine biomass producer due to its fast rate and well-established cultivation technology. A mixed culture system was established by inoculating it with sludge from an anaerobic digester, and then operated in a continuous mode for over 1000 days by feeding it a non-sterile suspension of dried L. japonica in tap water without additional nutrients. The average yield and productivity of total organic acids during the stationary phase were as high as 0.47 g/g COD and 2.45 g/L/day at pH 7 with 84.08% of acetate in total organic acids. The volatile fatty acids (VFAs) from the continuously-stirred tank reactor (CSTR) were used by oleaginous yeast (Cryptococcus curvatus) to produce lipids in a repeated batch system. The highest lipid content was 61%. The composition of the fatty acids was quite similar to that of vegetable oils. This system has potential application to biodiesel production. (C) 2014 Elsevier B.V. All rights reserved.
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