期刊
PEST MANAGEMENT SCIENCE
卷 -, 期 -, 页码 -出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.7664
关键词
Ralstonia solanacearum; bacterial wilt; type III secretion system (T3SS); inhibitor; mandelic acid derivatives; mechanism
A series of mandelic acid derivatives containing 2-mercapto-1,3,4-thiazole moiety were synthesized as potential inhibitors of Ralstonia solanacearum type III secretion system (T3SS). Among them, compound F-24 significantly inhibited the transcription of hrpY gene and attenuated hypersensitive response (HR) without inhibiting bacterial growth. F-24 showed better inhibitory activity than previously reported inhibitors and had the potential to control R. solanacearum.
BACKGROUNDBacterial wilt induced by Ralstonia solanacearum is regarded as one of the most devastating diseases. However, excessive and repeated use of the same bactericides has resulted in development of bacterial resistance. Targeting bacterial virulence factors, such as type III secretion system (T3SS), without inhibiting bacterial growth is a possible assay to discover new antimicrobial agents. RESULTSIn this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2-mercapto-1,3,4-thiazole moiety was synthesized. One of them, F-24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F-24 in protecting plants demonstrated that F-24 protected tomato plants against bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR-PhcA-PrhG-HrpB pathway. CONCULSIONThe screened F-24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F-24 is a compound with good potential in the control of R. solanacearum. & COPY; 2023 Society of Chemical Industry.
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