期刊
MOLECULAR PLANT PATHOLOGY
卷 17, 期 9, 页码 1398-1408出版社
WILEY-BLACKWELL
DOI: 10.1111/mpp.12376
关键词
D. zeae; regulation; rice foot rot; SlyA; zeamines
资金
- National Basic Research Program of China (973 Program) [2015CB150600]
- National Natural Science Foundation of China [31270170, 31201479, 31470236]
Dickeya zeae is a causal agent of rice root rot disease. The pathogen is known to produce a range of virulence factors, including phytotoxic zeamines and extracellular enzymes, but the mechanisms of virulence regulation remain vague. In this study, we identified a SlyA/MarR family transcription factor SlyA in D. zeae strain EC1. Disruption of slyA significantly decreased zeamine production, enhanced swimming and swarming motility, reduced biofilm formation and significantly decreased pathogenicity on rice. Quantitative polymerase chain reaction (qPCR) analysis confirmed the role of SlyA in transcriptional modulation of a range of genes associated with bacterial virulence. In trans expression of slyA in expI mutants recovered the phenotypes of motility and biofilm formation, suggesting that SlyA is downstream of the acylhomoserine lactone-mediated quorum sensing pathway. Taken together, the findings from this study unveil a key transcriptional regulatory factor involved in the modulation of virulence factor production and overall pathogenicity of D. zeae EC1.
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