期刊
MOLECULAR PLANT PATHOLOGY
卷 17, 期 7, 页码 1111-1123出版社
WILEY
DOI: 10.1111/mpp.12366
关键词
frameshift; gene expression; overlapping gene; RNA silencing suppression; RNA virus; transcriptional slippage
资金
- Wellcome Trust [088789, 106207]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J007072/1, BB/J015652/1]
- Academy of Finland [1134335, 1276136]
- Consultative Group for International Agricultural Research (CGIAR) consortium research programme for roots, tubers and bananas
- BBSRC [BB/J007072/1, BB/J015652/1] Funding Source: UKRI
- Wellcome Trust [106207/Z/14/Z] Funding Source: Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/J015652/1, BB/J007072/1] Funding Source: researchfish
- Wellcome Trust [106207/Z/14/Z] Funding Source: researchfish
The single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3 third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA(6) sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA(6) sequence, with higher slippage efficiency (approximate to 5%) than at the pipo site (approximate to 1%). Transient expression of recombinant P1 or the transframe' product, P1N-PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N-PISPO inhibited short-distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co-opted for the evolution and expression of further novel gene products.
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