期刊
OPTICS AND LASERS IN ENGINEERING
卷 167, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.optlaseng.2023.107604
关键词
Confocal Raman microscope; Fast axial focusing; In situ topography imaging and Raman mapping
类别
Non-axial scanning confocal Raman microscopy (NASCRM) is proposed to achieve high-precision topography imaging and Raman mapping by constructing an axial focusing system with a large range and high precision. Compared with CRM using confocal microscopy (CM) focusing, NASCRM improves the axial focusing resolution by 5.3 times and the Raman mapping speed by 2.9 times. This study provides a novel method for both conventional CRM and CRM-based imaging in various fields.
Confocal Raman microscopy (CRM) is extensively employed in numerous fields because it provides molecular fingerprint information without destroying or labeling samples. For samples with large topographic fluctuations, existing technology requires axial scanning to achieve axial focusing, which further increases the time required for Raman mapping. Herein, a method called non-axial scanning confocal Raman microscopy (NASCRM), which constructs an axial focusing system with a large range , high precision by modifying the wide-field imaging system, is proposed. The axial focusing system combined with CRM can simultaneously realize in situ high-precision topography imaging and Raman mapping. Theoretical analyses and preliminary experiments reveal that the axial focusing resolution of NASCRM is approximately 3 nm and the sensing range is approximately 60 mu m. Compared with CRM using confocal microscopy (CM) focusing (CM-CRM), the speeds within sensing range for axial focusing and Raman mapping are improved by 5.3 and 2.9 times, respectively. This study provides a novel method for both conventional CRM and CRM-based imaging in various fields.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据