期刊
MOLECULAR PLANT
卷 9, 期 7, 页码 961-974出版社
CELL PRESS
DOI: 10.1016/j.molp.2016.04.009
关键词
CRISPR/Cas9; genome editing; multiplex editing; mutation; plants
资金
- Guangdong Province Public Interest Research and Capacity Building Special Fund [2015B020201002]
- Ministry of Agriculture of the People's Republic of China [2014ZX08010-001, 2014ZX08009002]
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II adaptive immunity system. The CRISPR/Cas9 tool surpasses other programmable nucleases, such as ZFNs and TALENs, for its simplicity and high efficiency. Various plant-specific CRISPR/Cas9 vector systems have been established for adaption of this technology to many plant species. In this review, we present an overview of current advances on applications of this technology in plants, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing the induced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISPR/Cas9 systemin plants. In addition, we provide a perspective on the challenges of CRISPR/Cas9 technology and its significance for basic plant research and crop genetic improvement.
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