4.8 Article

β-Arrestin-independent endosomal cAMP signaling by a polypeptide hormone GPCR

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Pharmacology & Pharmacy

Characterisation of agonist signalling profiles and agonist-dependent antagonism at PACAP-responsive receptors: Implications for drug discovery

Zoe Tasma et al.

Summary: The PACAP-responsive receptors exhibit varied pharmacological profiles but activate signaling in a similar manner. The PAC(1s) receptor can act as a dual receptor for VIP and PACAP. The effectiveness of blocking a signaling pathway can be influenced by the endogenous PACAP family agonist present, with PACAP-38 showing less antagonism compared to PACAP-27 and VIP.

BRITISH JOURNAL OF PHARMACOLOGY (2022)

Article Biochemistry & Molecular Biology

Structural basis of sphingosine-1-phosphate receptor 1 activation and biased agonism

Zhenmei Xu et al.

Summary: This study reveals that p-arrestin-biased ligands direct a distinct activation path in S1PR1 through extensive interplay between the PIF and the NPxxY motifs, with key features including the intermediate flipping of W269(6.48) and the retained interaction between F265(6.44) and N307(7.49). The structural insights provided in this study offer a rational basis for designing novel signaling-biased S1PR modulators.

NATURE CHEMICAL BIOLOGY (2022)

Article Multidisciplinary Sciences

GPCR kinase knockout cells reveal the impact of individual GRKs on arrestin binding and GPCR regulation

J. Drube et al.

Summary: GPCR kinases (GRKs) play a regulatory role in GPCR interactions and functions. This study demonstrates that selective activation of GRKs leads to the formation of distinct GPCR-beta-arrestin complexes. The authors utilized GRK knockout cells to investigate the specific recruitment of beta-arrestins by different GRKs. Their findings provide insights into the detailed mechanisms of GRK-specific GPCR regulation and beta-arrestin complex formation.

NATURE COMMUNICATIONS (2022)

Article Multidisciplinary Sciences

Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias

Kouki Kawakami et al.

Summary: This study uncovers the role of Gq heterotrimer in determining GRK subtype selectivity, which in turn regulates the conformation and functionality of β-arrestin. By investigating the angiotensin II type-1 receptor, the authors find that both GRK2/3 and GRK5/6 are recruited by Ang II, while only GRK5/6 is recruited by the β-arrestin-biased ligand TRV027. Additionally, inhibition of Gq shifts the GRK subtype selectivity and β-arrestin functionality to that of TRV027.

NATURE COMMUNICATIONS (2022)

Article Multidisciplinary Sciences

OpenCell: Endogenous tagging for the cartography of human cellular organization

Nathan H. Cho et al.

Summary: This article introduces a method that uses various techniques to systematically map the localization and interactions of human proteins, and discovers the rich functional information contained in protein localization patterns.

SCIENCE (2022)

Article Biochemistry & Molecular Biology

State-selective modulation of heterotrimeric G?s signaling with macrocyclic peptides

Shizhong A. Dai et al.

Summary: This article explores a chemical library of 1012 cyclic peptides and identifies two macrocyclic peptides, GN13 and GD20, that can antagonize the activity and inactive states of the key protein Gas in the G protein-coupled receptor cascade. These macrocyclic peptides have high selectivity and specificity and can modulate cellular signaling through binding to crystallographically defined pockets.
Article Biochemistry & Molecular Biology

Membrane phosphoinositides regulate GPCR-(3-arrestin complex assembly and dynamics

John Janetzko et al.

Summary: This study reveals the role of membrane phosphoinositides (PIPs) in β-arrestin recruitment and GPCR-β-arrestin complex dynamics through cell-based and in vitro biophysical assays. GPCRs are classified into two groups, with one requiring PIP binding for β-arrestin recruitment and the other not. Plasma membrane PIPs enhance the active conformation of β-arrestin and stabilize GPCR-β-arrestin complexes by promoting a fully engaged state. As allosteric modulators of GPCR-β-arrestin complex dynamics, membrane PIPs allow for additional conformational diversity beyond GPCR phosphorylation alone. For GPCRs that require membrane PIP binding for β-arrestin recruitment, this provides a mechanism for rapid β-arrestin release upon GPCR translocation to endosomes, enabling its quick recycling.
Review Pharmacology & Pharmacy

Arrestin-Dependent and -Independent Internalization of G Protein-Coupled Receptors: Methods, Mechanisms, and Implications on Cell Signaling

Ee Von Moo et al.

Summary: This article discusses the importance of agonist-induced endocytosis in regulating cell surface receptor density and signaling. While arrestins are key regulators in GPCR internalization, there are also independent endocytosis pathways for GPCRs, expanding the diversity of the process.

MOLECULAR PHARMACOLOGY (2021)

Article Biochemistry & Molecular Biology

Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation

Alex D. White et al.

Summary: The study showed that similar amounts of cAMP generated by PTHR for similar lengths of time in different cellular locations, plasma membrane and endosomes, mediate distinct physiological responses. This suggests that subcellular signaling location plays a crucial role in achieving specificity in PTHR-mediated biological outcomes and may lead to rational drug design based on spatiotemporal manipulation of GPCR signaling.

SCIENCE SIGNALING (2021)

Article Biochemical Research Methods

CellProfiler 4: improvements in speed, utility and usability

David R. Stirling et al.

Summary: This study introduces CellProfiler 4, a new version of image analysis software with improved user interface and expanded functionality based on user feedback. Performance enhancements and optimizations have been made for large-scale analysis pipelines, providing researchers with significantly improved computational tools for extracting biological information effectively.

BMC BIOINFORMATICS (2021)

Article Biochemistry & Molecular Biology

Endosomal cAMP production broadly impacts the cellular phosphoproteome

Nikoleta G. Tsvetanova et al.

Summary: Endosomal signaling downstream of GPCRs plays a significant role in cellular responsiveness to cAMP, leading to distinct changes in phosphorylation reactions. A subset of proteins undergo dephosphorylation in response to cAMP, with compartmentalized PP2A activation by cAMP-responsive kinases as the likely mechanism.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Cell Biology

Accurate measurement of fast endocytic recycling kinetics in real time

Caspar T. H. Jonker et al.

JOURNAL OF CELL SCIENCE (2020)

Review Biochemistry & Molecular Biology

Structural insights into emergent signaling modes of G protein-coupled receptors

Ieva Sutkeviciute et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2020)

Review Pharmacology & Pharmacy

GPCR Signaling Regulation: The Role of GRKs and Arrestins

Vsevolod V. Gurevich et al.

FRONTIERS IN PHARMACOLOGY (2019)

Article Biochemistry & Molecular Biology

Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex

Anthony H. Nguyen et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2019)

Review Cell Biology

The subcellular dynamics of GPCR signaling

Davide Calebiro et al.

MOLECULAR AND CELLULAR ENDOCRINOLOGY (2019)

Article Biochemistry & Molecular Biology

Mini G protein probes for active G protein-coupled receptors (GPCRs) in live cells

Qingwen Wan et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2018)

Review Biochemistry & Molecular Biology

Structure and dynamics of GPCR signaling complexes

Daniel Hilger et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2018)

Review Biochemistry & Molecular Biology

Structure and dynamics of GPCR signaling complexes

Daniel Hilger et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2018)

Review Biochemistry & Molecular Biology

The Molecular Basis of G Protein-Coupled Receptor Activation

William I. Weis et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87 (2018)

Article Biotechnology & Applied Microbiology

Trends in GPCR drug discovery: new agents, targets and indications

Alexander S. Hauser et al.

NATURE REVIEWS DRUG DISCOVERY (2017)

Article Multidisciplinary Sciences

Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis

Thomas J. Cahill et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Article Multidisciplinary Sciences

Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

Amod Godbole et al.

NATURE COMMUNICATIONS (2017)

Article Biochemistry & Molecular Biology

GPCR-G Protein-β-Arrestin Super-Complex Mediates Sustained G Protein Signaling

Alex R. B. Thomsen et al.

Article Endocrinology & Metabolism

Persistent cAMP Signaling by Internalized LH Receptors in Ovarian Follicles

Sandra Lyga et al.

ENDOCRINOLOGY (2016)

Article Biochemistry & Molecular Biology

Spatially Restricted G Protein-coupled Receptor Activity via Divergent Endocytic Compartments

Frederic Jean-Alphonse et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Article Multidisciplinary Sciences

Visualization of arrestin recruitment by a G-protein-coupled receptor

Arun K. Shukla et al.

NATURE (2014)

Article Biochemistry & Molecular Biology

Endosomal GPCR signaling turned off by negative feedback actions of PKA and v-ATPase

Alexandre Gidon et al.

NATURE CHEMICAL BIOLOGY (2014)

Article Biochemistry & Molecular Biology

Spatial encoding of cyclic AMP signaling specificity by GPCR endocytosis

Nikoleta G. Tsvetanova et al.

NATURE CHEMICAL BIOLOGY (2014)

Article Endocrinology & Metabolism

Glucagon-like peptide-1 receptor-mediated endosomal cAMP generation promotes glucose-stimulated insulin secretion in pancreatic β-cells

Ramya S. Kuna et al.

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2013)

Review Pharmacology & Pharmacy

Structure-Function of the G Protein-Coupled Receptor Superfamily

Vsevolod Katritch et al.

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, VOL 53, 2013 (2013)

Article Biochemistry & Molecular Biology

Noncanonical Control of Vasopressin Receptor Type 2 Signaling by Retromer and Arrestin

Timothy N. Feinstein et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2013)

Article Multidisciplinary Sciences

Conformational biosensors reveal GPCR signalling from endosomes

Roshanak Irannejad et al.

NATURE (2013)

Article Multidisciplinary Sciences

Noncanonical GPCR signaling arising from a PTH receptor-arrestin-Gβγ complex

Vanessa L. Wehbi et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Biochemical Research Methods

Fiji: an open-source platform for biological-image analysis

Johannes Schindelin et al.

NATURE METHODS (2012)

Article Pharmacology & Pharmacy

Molecular Mechanism of Selectivity among G Protein-Coupled Receptor Kinase 2 Inhibitors

David M. Thal et al.

MOLECULAR PHARMACOLOGY (2011)

Article Biochemistry & Molecular Biology

Retromer terminates the generation of cAMP by internalized PTH receptors

Timothy N. Feinstein et al.

NATURE CHEMICAL BIOLOGY (2011)

Article Multidisciplinary Sciences

Structural flexibility of the Gas α-helical domain in the β2-adrenoceptor Gs complex

Gerwin H. Westfield et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)

Article Biochemistry & Molecular Biology

Sustained cyclic AMP production by parathyroid hormone receptor endocytosis

Sebastien Ferrandon et al.

NATURE CHEMICAL BIOLOGY (2009)

Article Biochemistry & Molecular Biology

Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors

Davide Calebiro et al.

PLOS BIOLOGY (2009)

Review Pharmacology & Pharmacy

Regulation of GPCRs by Endocytic membrane trafficking and its potential implications

Ayfin C. Hanyaloglu et al.

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY (2008)

Article Endocrinology & Metabolism

The human thyrotropin receptor is predominantly internalized by β-arrestin 2

Romy Frenzel et al.

ENDOCRINOLOGY (2006)

Article Biochemistry & Molecular Biology

Vasoactive intestinal polypeptide type-1 receptor regulation - Desensitization, phosphorylation, and sequestration

MA Shetzline et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2002)

Article Multidisciplinary Sciences

Multiple endocytic pathways of C protein-coupled receptors delineated by GIT1 sensitivity

A Claing et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)