期刊
MOLECULAR NEUROBIOLOGY
卷 54, 期 9, 页码 6984-6998出版社
HUMANA PRESS INC
DOI: 10.1007/s12035-016-0219-2
关键词
Ischemia-reperfusion injury; Ischemic preconditioning; Delayed neuronal death; Hypoxia inducible factor-1 alpha; Vascular endothelial growth factor; Nuclear factor-kappa B
资金
- Bio & Medical Technology Development Program of the NRF - Korean government, MSIP [NRF-2015M3A9B6066835]
- National Research Foundation of Korea [NRF-2013M3A9B6046563]
- Ministry of Science, ICT, and Future Planning
- Ministry of Science, ICT and Future Planning through the National Research Foundation [NRF-2015M3A9C4076322]
- National Research Foundation of Korea [22A20130012425] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ischemic preconditioning (IPC) provides neuroprotection against subsequent severe ischemic insults by specific mechanisms. We tested the hypothesis that IPC attenuates post-ischemic neuronal death in the gerbil hippocampal CA1 region (CA1) throughout hypoxia inducible factor-1 alpha (HIF-1 alpha) and its associated factors such as vascular endothelial growth factor (VEGF) and nuclear factor-kappa B (NF-kappa B). Lethal ischemia (LI) without IPC increased expressions of HIF-1 alpha, VEGF, and p-I kappa B-alpha (/and translocation of NF-kappa B p65 into nucleus) in CA1 pyramidal neurons at 12 h and/or 1-day post-LI; thereafter, their expressions were decreased in the CA1 pyramidal neurons with time and newly expressed in non-pyramidal cells (pericytes), and the CA1 pyramidal neurons were dead at 5-day post-LI, and, at this point in time, their immunoreactivities were newly expressed in pericytes. In animals with IPC subjected to LI (IPC/LI)-group), CA1 pyramidal neurons were well protected, and expressions of HIF-1 alpha, VEGF, and p-I kappa B-alpha (/and translocation of NF-kappa B p65 into nucleus) were significantly increased compared to the sham-group and maintained after LI. Whereas, treatment with 2ME2 (a HIF-1 alpha inhibitor) into the IPC/LI-group did not preserve the IPC-mediated increases of HIF-1 alpha, VEGF, and p-I kappa B-alpha (/and translocation of NF-kappa B p65 into nucleus) expressions and did not show IPC-mediated neuroprotection. In brief, IPC protected CA1 pyramidal neurons from LI by upregulation of HIF-1 alpha, VEGF, and p-I kappa B-alpha expressions. This study suggests that IPC increases HIF-1 alpha expression in CA1 pyramidal neurons, which enhances VEGF expression and NF-kappa B activation and that IPC may be a strategy for a therapeutic intervention of cerebral ischemic injury.
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