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H3K36 methylation maintains cell identity by regulating opposing lineage programmes

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NATURE CELL BIOLOGY
卷 25, 期 8, 页码 1121-+

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NATURE PORTFOLIO
DOI: 10.1038/s41556-023-01191-z

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Hoetker et al. demonstrate the dual role of H3K36 methylation in cell identity maintenance: integrating TGF-β signals to keep mesenchymal targets active and preventing the activation of alternative lineage programs through enhancer methylation. This study uncovers the crucial role of H3K36 methylation in maintaining cell identities across different developmental contexts.
Hoetker et al. show that H3K36 methylation exerts a dual role in cell identity maintenance: it integrates TGF & beta; signals at mesenchymal targets to keep them active and prevents the activation of alternative lineage programmes via enhancer methylation. The epigenetic mechanisms that maintain differentiated cell states remain incompletely understood. Here we employed histone mutants to uncover a crucial role for H3K36 methylation in the maintenance of cell identities across diverse developmental contexts. Focusing on the experimental induction of pluripotency, we show that H3K36M-mediated depletion of H3K36 methylation endows fibroblasts with a plastic state poised to acquire pluripotency in nearly all cells. At a cellular level, H3K36M facilitates epithelial plasticity by rendering fibroblasts insensitive to TGF & beta; signals. At a molecular level, H3K36M enables the decommissioning of mesenchymal enhancers and the parallel activation of epithelial/stem cell enhancers. This enhancer rewiring is Tet dependent and redirects Sox2 from promiscuous somatic to pluripotency targets. Our findings reveal a previously unappreciated dual role for H3K36 methylation in the maintenance of cell identity by integrating a crucial developmental pathway into sustained expression of cell-type-specific programmes, and by opposing the expression of alternative lineage programmes through enhancer methylation.

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