☆ 4.8 Editorial Material

Soft-landing methods aim to simplify structural biology

NATURE (2023)

Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography

Hannah Ochner et al.

Summary: Low-energy electron holography (LEEH) can explore the conformational space of proteins and distinguish different conformations of transferrin, indicating its viability for imaging function-related conformational changes in individual proteins.

SCIENTIFIC REPORTS (2023)

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Article Multidisciplinary Sciences

Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry

Michael S. Westphall et al.

Summary: This article introduces a method that integrates mass spectrometry and electron microscopy by depositing protein complex ions onto EM grids. The study shows that non-covalent protein complexes can retain their structure even after ionization and vaporization.

NATURE COMMUNICATIONS (2022)

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Article Multidisciplinary Sciences

Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition

Tim K. Esser et al.

Summary: Despite advances in cryo-EM and SPA, sample heterogeneity remains a challenge. The study demonstrates the potential of native ES-IBD as an alternative approach for preparing high-purity samples. This method has the potential to increase the scope and throughput of cryo-EM for protein structure determination.

PNAS NEXUS (2022)

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Article Multidisciplinary Sciences