期刊
MYCOSES
卷 66, 期 10, 页码 906-910出版社
WILEY
DOI: 10.1111/myc.13630
关键词
dermatophyte; Drosophila melanogaster; systemic infection
This study validates Drosophila melanogaster flies as a suitable model to study dermatophytic infections. It shows that wild-type flies are resistant to infection, while Toll-deficient flies are susceptible. Antifungal drugs can protect flies from infection, except for one specific fungus.
Background: Dermatophytosis is one of the most common fungal infections worldwide. The distribution of dermatophytes varies across continents, but the genera Trichophyton and Microsporum have emerged as the main isolated agents in humans and animals.Objectives: To validate Drosophila melanogaster flies as a fast and feasible model to study dermatophytic infections.Methods: Wild-type (WT) and Toll-deficient D. melanogaster flies were infected by Trichophyton rubrum, T. mentagrophytes, Microsporum canis and Nannizzia gypsea by pricking with a needle previously dipped in inoculum concentrations ranging from 10(3) to 10(8) colony-forming units/mL. Establishment of infection was confirmed by survival curves, histopathological analysis and fungal burden. Thereafter, flies were treated with terbinafine, itraconazole and clioquinol.Results: WT flies were predominantly resistant to the infection, whereas Toll-deficient flies succumbed to the four dermatophyte genera tested. The antifungal drugs protected flies from the infection, except for N. gypsea whose survival curves did not differ from the untreated group.Conclusions: This pilot study confirms that D. melanogaster is a suitable model to study the virulence and antifungal drug efficacy in dermatophyte species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据