期刊
MOLECULAR PHARMACEUTICS
卷 20, 期 9, 页码 4640-4648出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.3c00370
关键词
molecular spherical nucleic acids; PET-imaging; nanoparticles; delivery
F-18-labeled [60]fullerene-based molecular spherical nucleic acids (MSNAs), specifically synthesized and administered intravenously to HER2 expressing HCC1954 tumor-bearing mice, were monitored in vivo using PET/CT imaging. MSNA-PS, with improved enzymatic stability compared to MSNA-PO, exhibited an intriguing biodistribution profile in the body.
F-18-Labeled [60]fullerene-based molecularsphericalnucleic acids (MSNAs), consisting of a human epidermal growth factorreceptor 2 (HER2) mRNA antisense oligonucleotide sequence with a nativephosphodiester and phosphorothioate backbone, were synthesized, site-specificallylabeled with a positron emitting fluorine-18 and intravenously administratedvia tail vein to HER2 expressing HCC1954 tumor-bearing mice. The biodistributionof the MSNAs was monitored in vivo by positron emissiontomography/computed tomography (PET/CT) imaging. MSNA with a nativephosphodiester backbone (MSNA-PO) was prone to rapid nuclease-mediateddegradation, whereas the corresponding phosphorothioate analogue (MSNA-PS)with improved enzymatic stability showed an interesting biodistributionprofile in vivo. One hour after the injection, majorityof the radioactivity was observed in spleen and liver but also inblood with an average tumor-to-muscle ratio of 2. The prolonged radioactivityin blood circulation may open possibilities to the targeted deliveryof the MSNAs.
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