期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.202300401
关键词
autophagy; apoptosis; embryonic development; high-salt diet; mitochondrial function; oocyte maturation
This study suggests that a high-salt diet may impair female fertility in mice by weakening oocyte quality. The underlying mechanisms involve the inhibition of oocyte autophagy and apoptosis, as well as the disruption of mitochondrial function.
Scope: High salinity has been reported to induce many human disorders in tissues and organs to interfere with their normal physiological functions. However, it is unknown how salinity affects the development of female germ cells. This study suggests that a high-salt diet (HSD) may weaken oocyte quality to impair female fertility in mice and investigates the underlying mechanisms.Methods and results: C57BL/6 female mice are fed with a regular diet (Control) or a high-salt diet (HSD). Oocyte maturation, fertilization rate, embryonic development, and female fertility are evaluated. In addition, the spindle organization, actin polymerization, and kinetochore-microtubule attachment of oocytes are examined in both groups. Moreover, single-cell transcriptome data are used to demonstrate how HSD alters the transcript levels of genes. The observations confirm that HSD leads to female subfertility due to the deterioration of oocyte and embryo quality. The mechanism underlying reveals HSD compromises the oocytes' autophagy, apoptosis level, and mitochondrial function.Conclusion: The work illustrates that a high concentration of salt diet results in oocyte meiotic arrest, fertilization failure, and early developmental defection that embryos undergo to reduce female fertility in mice by perturbing the level of autophagy and apoptosis, mitochondrial function in oocytes.
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