Analysis of Coconut cadang-cadang viroid variants on field samples exhibiting variation in orange spotting symptom expression and severity

Article Food Science & Technology

COMPARISON OF REAL-TIME PCR, CONVENTIONAL PCR AND RT-LAMP FOR THE DETECTION OF COCONUT CADANG-CADANG VIROID VARIANT IN OIL PALM

Nur Diyana Roslan et al.

Summary: This study developed a real-time PCR method for the detection of oil palm Coconut Cadang-cadang Viroid (CCCVd) and compared its sensitivity and reliability with RT-LAMP and conventional PCR. The results showed that real-time PCR had higher sensitivity and reliability compared to the other two techniques, and the primers designed for real-time PCR were specific for detecting CCCVd variants.

JOURNAL OF OIL PALM RESEARCH (2023)

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Review Microbiology

Virus and Viroid-Derived Small RNAs as Modulators of Host Gene Expression: Molecular Insights Into Pathogenesis

S. V. Ramesh et al.

Summary: Virus-derived siRNAs play a crucial role in plant defense response, while pathogen infection can lead to the production of sRNAs targeting host mRNAs and causing disease symptoms. These interactions between pathogen-derived sRNAs and host mRNAs provide insights into the molecular mechanisms of pathogenesis.

FRONTIERS IN MICROBIOLOGY (2021)

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Review Biochemistry & Molecular Biology

An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids

Srividhya Venkataraman et al.

Summary: Viroids are small single-stranded circular RNA pathogens that infect plants, causing symptoms without encoding any proteins. This review covers viroid classification, molecular biology, spread, pathogenesis, host interactions, detection, and future research prospects.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Review Multidisciplinary Sciences

Progress in 50 years of viroid research-Molecular structure, pathogenicity, and host adaptation

Teruo Sano

Summary: Viroids are non-encapsidated, single-stranded, circular RNAs that replicate autonomously in host cells, invading and adapting to new environments. They interact with various host factors to exert different levels of pathogenicity, revealing a molecular mechanism of pathogenicity more complex than initially thought.

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES (2021)

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Review Cell Biology