Characteristic of Przewalski horses population from Askania-Nova reserve based on genetic markers

Przewalski horses are considered the last living population of wild horses, however, they are secondarily feral offspring of herds domesticated similar to 5000 years ago by the Botai culture. After Przewalski horses were almost extinct at the beginning of the twentieth century, their population is about 2500 individuals worldwide, with one of the largest breeding centers in Askania-Nova Biosphere Reserve (Ukraine). The research aimed to establish the maternal variation of Przewalski horses population maintained in Askania-Nova Reserve based on mitochondrial DNA hypervariable 1 and hypervariable 2 regions profiling, as well as, analysis of Y chromosome single nucleotide polymorphism unique for Przewalski horses, and coat color markers: MC1R and TBX3. The mtDNA hypervariable regions analysis in 23 Przewalski horses allowed assigning them to three distinctly different haplotypes, showing the greatest similarity to the Equus caballus reference, the Equus przewalskii reference, and to extinct species-Haringtonhippus. The Y chromosome analysis using fluorescently labelled assays differentiated horses in terms of polymorphism (g731821T>C) characteristic of Equus przewalskii. All male individuals presented genotype C characteristics for Przewalski horses. The polymorphisms within the coat color genes indicated only native, wild genotypes. The Y chromosome and coat color analysis denied admixtures of the tested horses with other Equidae.

Automated summary

Przewalski horses are the last population of wild horses, descended from herds domesticated by the Botai culture 5000 years ago. This study aimed to determine the maternal variation of Przewalski horses in Askania-Nova Reserve through analysis of mitochondrial DNA and Y chromosome markers, as well as coat color genes. The results showed distinct haplotypes in the mtDNA analysis, unique Y chromosome polymorphisms, and confirmation of native, wild genotypes through coat color analysis.